Proteins tracked by fluorescence recovery after photobleaching (FRAP) showed that they are ALL mobile and NOT restricted in movement.  True  False

Q: Compute for the albumin to globulin ( A/G) ratio using these data given Albumin =51.9 g/L Total…

A: Different macromolecules are present in the body. They include carbohydrates, proteins, nucleic…

Q: 5. Coomassie Brilliant Blue G-250, a dye used in the Bradford assay, has a maximum absorbance at 595…

A: Proteins can be separated based on their size, net charge and affinity towards a particular ligand.

Q: This lab examines the relationship between the absorbance of light by a solution at 595 nm and the…

A: Bovine serum albumin or BSA is a serum albumin protein derived from cows. It is often used as a…

Q: What is the purpose of adding APS and TEMED

A: SDS-PAGE stands for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis is a biochemistry…

Q: Spectrophotometric determination of riboflavin at max 445 nm.

A: When Lab scientists have to be measured total protein by UV-spectroscopy some important things…

Q: calculate P1 for amino acid Tryptophan. Also please check if I draw the mechanism correctly and…

A: The proteins are constituted of 20 naturally occurring amino acids. The amino acid Tryptophan is an…

Q: What feature of proteins do each of these techniques utilize to identify or separates proteins: gel…

A: It is a laboratory technique for the separation of a mixture by using a solvent on a filter paper.

Q: When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient…

A: HPLC is expanded as High Performance Liquid Chromatography. It helps in excluding out the analyte in…

Q: mixed micron cuboidal shaped cells within the stronger paste (F127) and the physical/biochemical…

A: F127 is the pluronic F127. It is a type of block copolymer and is widely used in pharmaceuticals,…

Q: Wavelength vs. Absorbance UV - VIS Residual Graph 0.05 -0.05 -0.1 -0.15 -0.2 -0.25 220 240 260 280…

A: The residual plot shown here is Wavelength Vs Absorbance plot has unique pattern. Here, plot is…

Q: When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands…

A: Zymography, also known as substrate gel electrophoresis, is an experimental technique for…

Q: Do letter 4 and draw a titration curve as directed in letter 4 c) for pka 4.7, labelling all the…

A: 4. a) Ionization Reaction of Water and the Equilibrium Constant ExpressionWater (H₂O) can act as…

Q: Configurational entropy Molen globules Clusters of frustrated contacts Functionally inet states B…

A: Everything that exists in our universe, from the fundamental elementary particles like quarks and…

Q: True or false? the size of the pores of a polyavrymide gel decrease as the concentration of…

A: There are different techniques used for the electrophoresis of proteins, of which the most commonly…

Q: A drop of a solution containing a mixture of amino acids gly, ala, glu, arg, his was applied to the…

A: Amino acid that will move towards anode is Glutamic acid (Glu) because it is an acidic amino acid.

Q: W) Plot the "specific fluorescence” against time in a graph, and comment on the result.

A: Fluorescence is the phenomenon of emission of specific wavelengths of light by any fluorescent…

Q: Research Center received a protein sample in a liquid form from her colleague recently. However, the…

A:

Q: Specificity Specificity is the ability to measure accurately and specifically the analyte of…

A: To calculate the recovery percentage for each solution, you need to compare the peak areas obtained…

Q: how can phosphorylation be detected via 2D gels?

A: 2D gels It refers to the two-dimensional gel electrophoresis. It is generally used to detect and…

Q: True or false Explain your answer To observe the Bacterial species E. coli with bright field…

A: Bright field microscopy is a widely used technique in which specimens are observed under normal or…

Q: In SDS page, the ________ proteins will move through the gel fastest and first, and in size…

A: SDS-PAGE is biochemistry technique used to separate proteins based upon the relative mobility of…

Q: are all the protein separated properly using isoelectric focusing? why or why not?

A: The proteins are composed of amino acids that have ionizable side chains with characteristic pKa…

Q: Why is COVID-19 a man made? (Brief explanation only atleast 3-5 sentences)

A: Coronavirus is  large family of virus that are  the source of respiratory infection, including the…

Q: When assessing general analysis of protein concentration in food, absorbance at 280 nm vs…

A: Assessment of protein concentration in food is generally done by BCA assay or by Bradford method or…

Q: Alkaline phosphatase conjugated to the anti-DIG antibody recognizes the Dig-labeled probe. The…

A: Introduction  Alkaline phosphatase (AP) is an enzyme that can catalyze the hydrolysis of various…

Q: Why does a dual layer of Cr/Au (20/100nm) delaminate during a GSTAT electrochemical polymerization…

A: Galvanostatic Polymerization with a Static Template (GSTAT) is a polymerization technique used for…

Q: True or false?  each objective of a phase contrast microscope contains a phase plate.   most…

A: Microscopy is a technique that is used to visualize small-sized objects that are not visible to the…

Q: Is there any interaction between the peptides given below with their single letter abbreviations in…

A: Proteins are made up of amino acids that are linked together by peptide bonds. Usually, in the cell…

Q: Can you please help me to complete step by step the Log molecular weight I will appreciate your help

A: Proteins are polymers of amino acids linked by peptide/amide bonds by the condensation reaction…

Q: Assume one has a cellophane membrane with a pore size of 400 Daltons. Which of the following dyes…

A: Diffusion occurs when all particles (atoms and molecules) are in continual random motion and tend to…

Q: please solve this step by step calculations

A: Detailed explanation:1. Protein concentration in the original sampleIn an assay tube, you have…

Q: plot the graph

A: First open a new excel sheet and enter the values as entered in the figure.Next, click on 'Insert'…

Q: True or False: Beta sheets are helical structures

A: Proteins are the ultimate products of the genes. DNA is transcribed into m RNA and this is…

Q: Which known dye samples are positively charged? Which known dyes are in the unknown sample in…

A: Agarose Gel Electrophoresis is a crucial analytical technique employed for the separation of…

Q: "Regarding resolution of mass spectrometers and various ways mass of peptides can be represented,…

A: Mass spectrometry is a measured charge to mass ratio.

Q: the Marquis test was used on a sample suspected to contain amphetamine in a “field” sample, so the…

A: The colourimetric analysis involves the determination of a chemical substance (inorganic and…

Q: 1. Isoelectric focusing. A digested protein sample was applied to one end of a gel strip with an…

A: Isoelectric focusing is a technique used to separate proteins on the basis of their isoelectric…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: Calculate the estimated concentration of test agent that inhibit cell growth by 50% ( GI50) value

A: Temozolomide is a drug used to treat certain types of brain tumors. It belongs to a class of…

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