Proteins tracked by fluorescence recovery after photobleaching (FRAP) showed that they are ALL mobile and NOT restricted in movement.  True  False

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A: Proteins can be separated based on their size, net charge and affinity towards a particular ligand.

Q: What is the purpose of adding APS and TEMED

A: SDS-PAGE stands for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis is a biochemistry…

Q: Spectrophotometric determination of riboflavin at max 445 nm.

A: When Lab scientists have to be measured total protein by UV-spectroscopy some important things…

Q: mixed micron cuboidal shaped cells within the stronger paste (F127) and the physical/biochemical…

A: F127 is the pluronic F127. It is a type of block copolymer and is widely used in pharmaceuticals,…

Q: Wavelength vs. Absorbance UV - VIS Residual Graph 0.05 -0.05 -0.1 -0.15 -0.2 -0.25 220 240 260 280…

A: The residual plot shown here is Wavelength Vs Absorbance plot has unique pattern. Here, plot is…

Q: When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands…

A: Zymography, also known as substrate gel electrophoresis, is an experimental technique for…

Q: Configurational entropy Molen globules Clusters of frustrated contacts Functionally inet states B…

A: Everything that exists in our universe, from the fundamental elementary particles like quarks and…

Q: True or false? the size of the pores of a polyavrymide gel decrease as the concentration of…

A: There are different techniques used for the electrophoresis of proteins, of which the most commonly…

Q: W) Plot the "specific fluorescence” against time in a graph, and comment on the result.

A: Fluorescence is the phenomenon of emission of specific wavelengths of light by any fluorescent…

Q: 1 2 3 4 5 6 N Sample Lysate Name volume 8 Tilapia Cod Sole Perch Tilapia Cod Sole 2 μl Perch 10 με 2…

A: Protein sample: A non-branching sequence of amino acids joined together by a single peptide bond…

Q: how can phosphorylation be detected via 2D gels?

A: 2D gels It refers to the two-dimensional gel electrophoresis. It is generally used to detect and…

Q: True or false Explain your answer To observe the Bacterial species E. coli with bright field…

A: Bright field microscopy is a widely used technique in which specimens are observed under normal or…

Q: True or false?  each objective of a phase contrast microscope contains a phase plate.   most…

A: Microscopy is a technique that is used to visualize small-sized objects that are not visible to the…

Q: Can you please help me to complete step by step the Log molecular weight I will appreciate your help

A: Proteins are polymers of amino acids linked by peptide/amide bonds by the condensation reaction…

Q: Assume one has a cellophane membrane with a pore size of 400 Daltons. Which of the following dyes…

A: Diffusion occurs when all particles (atoms and molecules) are in continual random motion and tend to…

Q: please solve this step by step calculations

A: Detailed explanation:1. Protein concentration in the original sampleIn an assay tube, you have…

Q: plot the graph

A: First open a new excel sheet and enter the values as entered in the figure.Next, click on 'Insert'…

Q: Which known dye samples are positively charged? Which known dyes are in the unknown sample in…

A: Agarose Gel Electrophoresis is a crucial analytical technique employed for the separation of…

Q: "Regarding resolution of mass spectrometers and various ways mass of peptides can be represented,…

A: Mass spectrometry is a measured charge to mass ratio.

Q: 1. Isoelectric focusing. A digested protein sample was applied to one end of a gel strip with an…

A: Isoelectric focusing is a technique used to separate proteins on the basis of their isoelectric…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: You may want to use this resource for this problem. If you do, submit the output along with your…

A: The confocal microscope is an advanced imaging technique that allows for high-resolution and…

Q: COMPUTATIONAL TRUE OR FALSE Milk sample A contained an average of 53 cells per FOV at a dilution…

A: Given: Average cells, Sample A = 53 cells per FOV Sample B = 99 cells per FOV In order to get…

Q: A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish…

A: The largest organ of the body, the skin, is composed of water, protein, lipids, and minerals. It…

Q: Laemmli polyacrylamide gel electrophoresis (SDS-PAGE). The essence of the method. The role of SDS in…

A: SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis) is an electrophoresis method…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: How far each bands has run for A and B compared to that dye front ?

A: Electrophoresis is a laboratory technique used to separate DNA fragments based on their size and…

Q: state transition diagram for home nursing during covid-19

A: Those who are requiring form CoVid-19 might require home care due to their post CoVid weakness. This…

Q: Hand draw the expected gel result from the graphical data of three extracted RNA samples (in Figure…

A: Nanodrop spectrophotometer is a technique which helps in measuring the nucleic acid that is DNA/ RNA…

Q: True or false .Very powerful X-rays used in crystallography are generated in a very large facilities…

A: X-ray crystallography is a tool used to determine the composition of atoms and crystalline cells.…

Q: True or false?       light microscopy has a limit of resolution of 200nm.   phase contrast…

A: Microscope is an instrument used to visualize minute / microscopic things . There are various types…

Q: mobilitiy

A: Relative mobility id determined by measuring the distance from the top of the gel to the middle of…

Q: We performed two methods of electrophoresis this semester. List both electrophoresis methods, and…

A: Electrophoresis is the migration of charged particles under the influence of electric current.…

Q: MRI What does the "induced free decay" curve describe?

A: Magnetic Resonance Imaging (MRI) is a non-invasive medical imaging technique that uses a strong…

Q: Why are integral proteins more difficult to study then peripheral, and why integral can only be…

A: The ease with which a molecule can flow through a cell membrane is referred to as "permeability".…

Q: Fischer projection of xylitol (sweetening agent in "sugarless gum", candy,  and sweet creals.)

A: Fischer projection is a diagrammatic representation of an organic molecule that demonstrates a…

Q: Below is shown the FRAP profiles of three proteins X, Y and Z, which of the protein(s) is/are most…

A: FRAP stands for fluoresence recovery after photobeaching(FRAP) is a technique to study mobility of…

Q: Liquid supported membrane of DGA used for rare earth elements separation. Tell me the…

A: liquid-supported membrane of di-(2-ethylhexyl) phosphoric acid." It is a type of membrane used for…

Q: These questions regarding NMR. What is the purpose of NMR calibration? Predict the following…

A: Hi!.I am only answering for the  H1- NMR of first three compounds of your question since we are only…

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