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- A amp PBR322 4301 fot B Clear Zones Figure 2 The postgraduate student, Demika, inserted her gene of interest into the plasmid, pBR322, before transformation into the competent host cell using heat shock method. After that she cultured the cells on the Ampicillin agar plate before replica plating the colonies onto another Ampicillin (A) and Tetracycline (B) agar plates shown in Figure 2. (1) Referring to the vector pBR322 in Figure 2, which recognition site was cleaved to insert the gene of interest? Based on the observation above, can you identify which colonies are carrying positive mcombinants of BR322? Explain your selection.Section B: Circular DNA Plasmid map Eco01091 2674 Pfol 46 BSTAPI 179 Aatil 2617 Ndel 183 Sspl 2501 Ehel 235 Pdml 2294 146/ 236 lacz Bcgl 2215 Scal 2177 \2486 MCS 469 PUC18/19 2686 bp BsaXI 659 Sapl 683 852 AflI, Pscl 806 Gsul 1784 1626 Cfr101 1779. Eco311 1766 Eam11051 1694 1466 Гер (pMB1) BseYl 1110 \Cail 1217 Ed Acs CCT CCA GGT CGA CrC TAG AGG ATC CC GGG TAC CGA CCT CGA ATT CT MT CAT G07 CAT AGC TGT 7C CtG 3 ITA OTA CCA GTA TCI ACA ANG GC Y 3 C ATT TO Crs cOG Lacz Val Val Al Leu Ass The e et Thr Met 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp, What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl & Gsul b. Scal, Pdml & BsaXI c. Scal, Sspl & Ehel 2. You have determined that the amplicon you want to clone has enzyme restriction sites for Hindll and ECORI, After investigation you have seen that…13-14. A set of Hfr strains transfer markers in the following order. Strain Transfer order JIAF G 1 ED BGFA IJH CE D CED B 13. Which of the following gene orders is correct? A: AFGEDBCHIJ B: ECHIJAFGBD C: E D B G FACH JI D: E D B G FACHIJ E: A F G BD ECHJI 14. Which Hfr strains have F factors inserted in the same orientation? A: 1 and 3 oriented the same way, 2 and 4 both oriented the other way B: 1 and 2 oriented the same way, 3 and 4 both oriented the other way C: 1 and 4 oriented the same way, 2 and 3 both oriented the other way D: 1 oriented one way, 2, 3 and 4 all oriented the other way E: all are oriented the same way 2 UYSerlero
- esting 8MU376MpwkNtkrF5%252B26BVPP%252Bzg%253D%2. Question Image 1 3 6. 7. 8. with the figure? cracteristics ight differe dividuals can compared Q. Which of following can you do with the biotechnology depicted in the figure? • 0 1:47 Sign out || O|| | 11anline State- estid%3D1740&strandid%3D&element3D&difficulty=Dassessment&assignment_id%3D42985127&load_test=1&teacherPreview=#test Save Subm gp120 Docking Glycoprotein Lipid Membrane RNA gp41 Transmembrane Glycoprotein Сapsid Matrix Reverse Transcriptase Viruses, although not considered to be alive, attack host cells and cause disease. The attack of a host cell is necessary for the virus to survive for all of the reasons listed EXCEPT one. That is they cannot synthesize proteins becayse they lack ribosomes and must use the ribosomes of their host cells to translate viral messenger RNA into new Eliminate proteins. A) they cannot produce or store energy in the form of ATP and have to get their energy from the host cell. B) they parasitize the host cell for basic molecules like amino acids, nucleotides, and lipids. D) they lack DNA/RNA so they must use the DNA of the host cell. Address 立Practice Case study p. 224 part: Tuberculosis (TB) in indigenous populations. Lily was a recent science graduate of the University of Alberta and was 11 months into her 12 - month Master of Science in Global Health degree, at Dalhousie University. This relatively new program was created to provide the multidisciplinary training required of scientists working to meet the complex challenges of global public health issues. The University had partnered with the National Collaborating Centre for Indigenous Health (NCCIH) and that was why Lily now found herself doing her Global Health Field Practicum in the Northern Territories, working on a study of drug resistant TB infections among Innuit communities. The different communities included in her research were close to 7000 individuals, with multiple diagnosed cases of TB. And while she was grateful that drug-resistant TB in Canada remained below the global average (Public Health Agency of Canada, 2019), she was cognizant of the possibility…
- Is the GASP phenotype present? In LB + NaCl? Un-Aged Cells Aged Cells LB culture Day 0 LB + NaCl culture LB culture Day 14 LB + NaCl culture •Practice Case study p. 224 part: Tuberculosis (TB) in indigenous populations. Lily was a recent science graduate of the University of Alberta and was 11 months into her 12 - month Master of Science in Global Health degree, at Dalhousie University. This relatively new program was created to provide the multidisciplinary training required of scientists working to meet the complex challenges of global public health issues. The University had partnered with the National Collaborating Centre for Indigenous Health (NCCIH) and that was why Lily now found herself doing her Global Health Field Practicum in the Northern Territories, working on a study of drug resistant TB infections among Innuit communities. The different communities included in her research were close to 7000 individuals, with multiple diagnosed cases of TB. And while she was grateful that drug-resistant TB in Canada remained below the global average (Public Health Agency of Canada, 2019), she was cognizant of the possibility…Define/Compare/Contrast/Distinguish these three terms: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)
- Jm109 cells transformed with ligation and plasmid alone is used. The colonies most like: white blue light blue No coloniesdraw structure of plasmidAll of the following lead to horizontal gene transfer EXCEPT. O plasmids transfer of genetic material from parent to progeny transposable elements viral infection 0000