Since KOH can also determine whether a culture is Gram positive or negative, why go through all the trouble of doing a Gram stain instead? part 2 What causes the KOH test to work? -part 3 How do you know a KOH test has identified a gram negative? Gram positive?
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Q5) Since KOH can also determine whether a culture is Gram positive or negative, why go through all the trouble of doing a
part 2 What causes the KOH test to work?
-part 3 How do you know a KOH test has identified a gram negative? Gram positive?
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- Q5) Since KOH can also determine whether a culture is Gram positive or negative, why go through all the trouble of doing a Gram stain instead? - What causes the KOH test to work? - How do you know a KOH test has identified a gram negative? Gram positive?a. After conducting the Gram staining procedures and you observed that all cells are stained with too much purple though the bacteria you fixed is Gram negative, what could be the mistake you have done? b. After conducting the Gram staining procedures and you observed that all cells are stained with too much pink though the bacteria you fixed is Gram positive, what could be the mistake you have done?1) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step during the staining procedure. 2) What is a gram variable and why can gram variability occur?
- Q2) Why do cheek cells stain red with the gram stain? What is the big red circle in the middle of the cheek cell? - Considering that it isn’t possible to identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic? - What cells stain red in a Gram stain? What cells stain purple in a Gram stain?N Please observe the following cells that have been Gram stained. "* 3 E ER $ JO 4 R % 5 6 F G & Y I U 8 00Which step of the gram stain procedure is most critical?why?
- 1.why is gram stain considered a differential stain? 2.How do gram positive and gram negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties? 3.How does the age of a culture affect the gram stain reaction? What is an optimum culture age for a valid gram reaction? 4.Which step in the gram stain procedure is most prone to error?If done correctly how might that step affect the end result? 5.what is the function of mordant, and which reagent serves this purpose in the gram stain procedure? 6.List the reagents of the gram star technique in order and their general role in the staining process. 7.In what type of cell, gram -positive or gram-negative , would you find lipopolysaccharide in its cell wall?This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantSelect all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells