Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a hypothetical protein. The nucleotides are numbered 1 to 100.  a)Although the transcription start site begins at the underlined C/G, which of the following is the nucleotide sequences needed upstream for transcription to actually occur? b)What are the first 15 nucleotides of the mRNA? c)What are the first 5 amino acids translated from the resulting mRNA? d)A different mutation results in the substitution of the T/A base pair at position 30 (shown in bold and underlined) with a G/C base pair. How would this mutation affect the sequence of the protein that is produced?

Aquaculture Science
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ISBN:9781133558347
Author:Parker
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Chapter4: Management Practices For Finfish
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Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a hypothetical protein. The nucleotides are numbered 1 to 100. 

a)Although the transcription start site begins at the underlined C/G, which of the following is the nucleotide sequences needed upstream for transcription to actually occur?

b)What are the first 15 nucleotides of the mRNA?

c)What are the first 5 amino acids translated from the resulting mRNA?

d)A different mutation results in the substitution of the T/A base pair at position 30 (shown in bold and underlined) with a G/C base pair. How would this mutation affect the sequence of the protein that is produced?

1
20
40
5'-GTGTCCGTCTAATАТTGTGAGATGTTАТАТСССGCCGTCAACACCAТСАА-3'
------+
3'-САСAGGCAGATTATAACAСТСТАСААТАТтAGGGCGGCAGттсTGGTAGTT-5'
60
80
100
5'-АСAGGAТААТСGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC-3'
------+----------+---------+-
-+---------+
3'-TGTCCTАТТAGCGGACGACCCCGTTTСCGCCАСТТCСАТТТССАСААCGG-5'
Transcribed Image Text:1 20 40 5'-GTGTCCGTCTAATАТTGTGAGATGTTАТАТСССGCCGTCAACACCAТСАА-3' ------+ 3'-САСAGGCAGATTATAACAСТСТАСААТАТтAGGGCGGCAGттсTGGTAGTT-5' 60 80 100 5'-АСAGGAТААТСGCCTGCTGGGGCAAAGGCGGTGAAGGTAAAGGTGTTGCC-3' ------+----------+---------+- -+---------+ 3'-TGTCCTАТТAGCGGACGACCCCGTTTСCGCCАСТТCСАТТТССАСААCGG-5'
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