Sequence the steps to transfect and observe a fluorescent protein in a slice of the brain:

Q: What is plasmid? What is the purpose of plasmid and how is it important?

A: Nucleotides are the compounds that makeup DNA. A phosphate group and a sugar group, and a nitrogen…

Q: Why is it important to include a selective marker gene on a plasmid? O a) So that you can select…

A: Bacteria are common source of plasmids. For the sole purpose of Cloning experiments, new plasmids…

Q: Which of the following is an effective transfection strategy in plant cells? Mark all that apply…

A: Transfection is a technique for genetically altering cells by introducing foreign nucleic acids into…

Q: In bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select…

A: Bacterial transformation is an important method that introduces foreign bacteria into a cell.  The…

Q: You have constructed a cDNA library in a phagemid vector. Describe how you would screen the library…

A: Phagemid vectors are plasmid DNA [Deoxyribonucleic acid] containing a small portion of the genome of…

Q: a) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c)…

A: Enzymes are mainly used for the DNA manipulation or used in Recombinant DNA technology. there are…

Q: Which of the following is true about restriction endonucleases?a) Type I and II requires ATP to move…

A: Restriction endonucleases are the specific DNA sequences that cleaves the sugar-phosphate backbone…

Tissue regeneration & repair

Tissue regeneration involves the restoration of tissue components by regrowth of damaged tissues. In contrast, tissue repair involves patching of injured tissue rather than restoring it. It is the proliferative capacity of the cells that decides the amount of regeneration against the repair that would occur in the tissue. Both regeneration and repair depending on the duration of the injury and response to the inflammation.