Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Sequence the steps to transfect and observe a fluorescent protein in a slice of the brain:
* Place the slice of brain under the microscope and observe it.
* Wait for the expression of the fluorescent protein by the transfected cells.
* Harvest and culture a slice of brain.
* Electropore in the slice a plasmid coding for a fluorescent protein.
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Step 1 introduction
Transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.
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