Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Sequence the steps to transfect and observe a fluorescent protein in a slice of the brain: 

* Place the slice of brain under the microscope and observe it.

* Wait for the expression of the fluorescent protein by the transfected cells.

* Harvest and culture a slice of brain.

* Electropore in the slice a plasmid coding for a fluorescent protein.

 

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Step 1 introduction

Transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection.

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