Seawater was spiked with phosphate to achieve a quantifiable range of phosphate concentrations by spectroscopy. What components of the spectrophotometer or sample preparation could be changed that would improve detection limits?
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Seawater was spiked with phosphate to achieve a quantifiable range of phosphate concentrations by spectroscopy. What components of the spectrophotometer or sample preparation could be changed that would improve detection limits?
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- Exactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.Seawater was spiked with phosphate to achieve a quantifiable range of phosphate concentrations by the spectrophotometer. What components of the spectrophotometer or sample preparation could be changed that would improve detection limits?The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.
- A sample solution containing quinine was analysed by fluorescence spectroscopy. 5 mL of the sample solution was diluted with 0.1 M HCl to a final volume of 100 mL. The fluorescence of the solution was measured and gave a value of 54. A reference solution containing 0.086 mg/mL of quinine gave a fluorescence reading of 38. A blank solution gave a fluorescence reading of 21. Calculate the quinine content of the sample solution in mg/mL.5 What would the effect of increasing sample pressure (while maintaining a fixed cell length) have on an FTIR spectrum. By ”cell” I mean the chamber holding the gas.Quinine in a 1.553-g antimalarial tablet was dissolved in sufficient 0.10 M HCl to give 250 mL of solution.A 10.00-mL aliquot was then diluted to 50.00 mL with the acid. The fluorescence intensity for the dilutedsample at 347.5 nm provided a reading of 196 on an arbitrary scale. A standard 100-ppm quinine solutionregistered 125 when measured under conditions identical to those for the diluted sample. Calculate the massin milligrams of quinine in the tablet.
- As a forensic toxicologist, you have been given a task on determining amount of cadmium, mercury, chromium, copper and lead metal contents in medicinal herbs using flame atomic absorption spectroscopy (AAS). Hollow cathode lamp (HCL) was selected as radiation source for this analysis. The highest concentration came from lead element followed by chromium and copper, however cadmium and mercury were not detected in the sample. a) Based on your knowledge, conclude whether the above result is valid or not. b) If you are given an option of electrothermal or flame atomizer for above case study, determine which one is suitable and justify your answer.Atomic Spectroscopy (a) In terms of spectral shape, how are atomic spectra different from molecular spectra? What is the source of this difference? (b) Describe the decision-making process for picking a line for atomic emission analysis of a given analyte. (c) For quality assurance, spike recovery is not sufficient to establish confidence in an analytical procedure. Explain this statement. What should be done instead to demonstrate satisfactory performance of an instrumental method (including elemental analysis). (d) Find a literature example of atomic spectroscopy used for elemental analysis. State the operating conditions with sufficient detail to reproduce the analysis; Please answer very soon will give rating surely All questions complete Answer needed Please help meCo(II) was used as an internal standard to analyze a sample of Ti(IV) with atomic absorption spectroscopy (AAS). A standard mixture containing 1.22 ug Co/mL and 2.20 ug Ti/mL measured by AAS produced a signal-to-signal ratio of 2.35 Ti : 1.00 Co. A mixture was prepared by combining 6.00 mL of a Ti(IV) solution of unknown concentration with 4.00 mL.of a 12.4 ug/mL solution of Co(II). The absorbance of the mixture at the Ti(IV) wavelength was 0.104 and the absorbance at the Co(II) wavelength was 0.189. Determine the concentration, in moles per liter, of Ti(IV) in the original unknown solution. concentration: M Ti(IV)
- The ppm concentration of Pb2+ in a blood sample were measured with Spectrophotometry. 5.00 mL of a blood sample were taken and this sample gave a signal of 0.301 a.u.. Another 5.00 mL of a blood sample were mixed with 0.50 mL og 1.75 ppm Pb2+. Then, this mixture was diluted to 25.00 mL and this diluted mixture gave a signal of 0.406 a.u.. What is the ppm concentration of a blood sample?A student wanted to determine the level of lead in amoxicillin powder, so he transferred 148 mg of the powder to 200 ml volumetric flask and completed the volume volumetrically, then he transferred 5 ml of the obtained solution to 50 ml volumetric flask and completed the volume properly, and read the absorbance of the obtained solution on AAS. The reading was 0.48. and the calibration equation was: "Y =12.25 x- 0.05" (concentration microg./mL). ?"Calculate the content of lead in amoxicillin as "ppm 326.5.a O 605.0 .b O 60.5.c O 17.5.d O 584.7.e O أخل اختياريA student wanted to determine the level of lead in amoxicillin powder, so he transferred 148 mg of the powder to 200 ml volumetric flask and completed the volume volumetrically, then he transferred 5 ml of the obtained solution to 50 ml volumetric flask and completed the volume properly, and read the absorbance of the obtained solution on AAS. The reading was 0.48. and the calibration equation was: "Y =12.25 x- 0.05" (concentration microg./mL). Calculate the content of lead in amoxicillin as "ppm"? O a. 326.5 O b. 605.0
