A sample solution containing quinine was analysed by fluorescence spectroscopy. 5 mL of the sample solution was diluted with 0.1 M HCl to a final volume of 100 mL. The fluorescence of the solution was measured and gave a value of 54. A reference solution containing 0.086 mg/mL of quinine gave a fluorescence reading of 38. A blank solution gave a fluorescence reading of 21. Calculate the quinine content of the sample solution in mg/mL.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry
Section: Chapter Questions
Problem 9.16QAP
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A sample solution containing quinine was analysed by fluorescence spectroscopy.

5 mL of the sample solution was diluted with 0.1 M HCl to a final volume of 100 mL. The fluorescence of the solution was measured and gave a value of 54. A reference solution containing 0.086 mg/mL of quinine gave a fluorescence reading of 38. A blank solution gave a fluorescence reading of 21.

Calculate the quinine content of the sample solution in mg/mL.

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