Sampling COUNTS 2nd dilution 5; 6 3rd dilution 0; 0 240; 235 1st dilution 4th dilution 1st 2nd 3rd 0, 0 55; 60 TNTC 30: 35 250;245 TNCT TNTC TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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For letter A, pls ILLUSTRATE (create an illustration or drawing) the DILUTION SERIES of the problem just like the sample on the 2nd image. 

Please read the instructions carefully as I have already posted this twice and the experts just copy-pasted the answers from my first post. I don't want to waste another post question for this one. Again, I NEED AN ILLUSTRATION and not just the computation/explanation through words so I can properly visualize the problem.

WILL UPVOTE if I get what I need.

1 ml
Sample to
be counted
1 ml
1 ml
1 ml
1 ml
1 ml
9-ml
broth
1/106
(10-)
Total
1/10
1/100
1/103
1/104
1/105
dilution (10-1)
(10-9
(10-9)
(10-)
(10-)
Plate 1-ml samples
159
17
2
Too many colonies colonies colonies colonies colonies
to count
159 x 103
Plate Dilution
count factor
1.59 x 105
Cells (colony-forming
units) per milliliter of
original sample
Transcribed Image Text:1 ml Sample to be counted 1 ml 1 ml 1 ml 1 ml 1 ml 9-ml broth 1/106 (10-) Total 1/10 1/100 1/103 1/104 1/105 dilution (10-1) (10-9 (10-9) (10-) (10-) Plate 1-ml samples 159 17 2 Too many colonies colonies colonies colonies colonies to count 159 x 103 Plate Dilution count factor 1.59 x 105 Cells (colony-forming units) per milliliter of original sample
Helping tags: Biology, bacterial count, dilution, serial dilution
WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls
show complete solutions also for the computation part. Thank you.
1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to
determine the population of the culture, by transferring 25 ml of the suspension to 225
ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9
ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The
following table shows the results of the plating method.
Sampling
COUNTS
2nd dilution
3rd dilution
4th dilution
0,0
30: 35
250;245
1st dilution
1st
2nd
3rd
0; 0
240; 235
TNTC
55; 60
5; 6
TNTC
TNCT
TNTC
TNTC
a) Illustrate the dilution series used and label the final dilution of each dilution.
b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours.
Show all computations.
Transcribed Image Text:Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.
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