s] Draw a picture showing one scheme for creating dilutions of E. coli cells by 104, 105, and 10-6.Include what volumes of cells and diluent you would pipet at each stage, and label each step with theindividual dilution factor happening within that step.8.

Serial dilution is a method that includes stepwise dilution of a substance in a sterile solvent.…

Answered: s] Draw a picture showing one scheme…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Your colleague handed you a novel strain of coli that is purifying a protein with a 6xHisTag; they claim it is superior to the TOP10 cells you have been using. But even with a larger culture size, you discover that your protein yield—using the same Ni-NTA column—is quite low. You discover that the novel strain of Escherichia coli generates an unusually high quantity of dicarboxylic acids, a byproduct of the citric acid cycle that is recognized for its ability to function as an all-purpose metal chelator. What do you think the issue is with purifying IMAC protein with this new strain of E. coli?
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s] Draw a picture showing one scheme for creating dilutions of E. coli cells by 104, 105, and 10-6. Include what volumes of cells and diluent you would pipet at each stage, and label each step with the individual dilution factor happening within that step.