RI-a Compound tested A B C DEG1 lod bos 2g 1 Mutanf 1 3. 4 101 + a. What is the order of compounds A to E in the pathway? b. At which point in the pathway is each mutant blocked? c. Would a heterokaryon composed of double mutants 1,3 and 2,4 grow on a minimal medium? Would 1,3 and 3,4? Would 1,2 and 2,4 and 1,4? | | |++ | + 1+ +

please give anser asap

28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+) leu(-) thr(+) and used to transform a recipient of genotype cys(-) leu(+) thr(-). Initially, the treated recipient population is plated on a minimal medium supplemented with threonine. Many colonies are obtained. What are possible genotypes of these colonies? A. cys(-) leu(+) thr(+) B. cys(+) leu(+) thr(-) C. All are cys(+) and either + or - for leu and thr D. All are thr(+) and either + or - for cys and leu E. All are thr(-) cys(-) and either + or - - for CYS

I just don't know how to get the answer for C and D. Expecially for C, why F+died because of leu-?

Beer's Law to determine Protein Concentration You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be1.35. Calculate the molar and mass concentration of the purified protein if the extinction coefficientand molecular weight of p53 is 35,410 M-1 cm-1 and 43,653 Da, respectively (l = 1 cm).

Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…

step by step calculations please

8 of 15 3-6 Insert Plasmid GITAA In this diagram, name the enzyme that converts the DNA substrates on the left into the product on the right. O Terminal transferase O Phosphatase O Topoisomerase O Ligase O Glycosylase

You transform competent cells (70 000 cells/ml) with a plasmid containing an antibiotic resistance gene and spread 1 ml of the transformed cells onto plates that select for transformants. If 35 colonies grow, what is your transformation rate? а. cross out 0.0005% O b. cross out 0.05% cross out С. 20% d. 2000 cells/ml cross out е. You cannot calculate cross out transformation rate with these numbers.

FOR ACADEMIC PURPOSES ONLY - NOT FOR CLINICAL USE Test Details Gene Genotype Phenotype Alleles Tested CYP2C19 *2/*7 Poor Metabolizer *2, *3, *4, *48, *5, *6, *7, *8, *9, *17 CYP2D6 *1/4 Intermediate Metabolizer *2, *3, *4, *4M, "6, *7, *8, *9, *10, *12, *14A, *148, *17, *29, *35, *41 CYP3AS *3/*3 Poor Metabolizer *1D, *2, *3, *38, *3C, "6, *7, "8, *9 CYP3A4 *1/*1 Normal Metabolizer *18, *2, *3, *12, *17, *22 VKORCI -1639G>A G/A Intermediate Warfarin Sensitivity -1639G>A *2, *3 *2, *3, *4, *5, "6, *11 CYP4F2 *1/3 Decreased Function CYP2C9 *1/*1 Normal Metabolizer CYP2B6 *1/*1 Normal Metabolizer *6, *9 Normal Metabolizer - Higher Inducibility CYPIA2 *1F/"1F *1C, *1D, *1F, *1K, *1L, *1V, *1W SLCOIB1 521T>CT/T Normal Function 521T>C, 388A>G CFTR F508del/RS53X Negative Numerous DPYD *1/*1 Normal Metabolizer Numerous ТРМТ *1/*3A *1/1 Intermediate Metabolizer Normal Metabolizer *2, *3A, *38, *3C, *4 *2, *3, *4, *5, "6, *7, *8, *9 NUDT15 UGTIAI *1/*36 Normal Metabolizer *6, *27, *28, *36, *37,…