Chemistry
Chemistry
10th Edition
ISBN: 9781305957404
Author: Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher: Cengage Learning
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Experiment 2 Chromatography
2. A student properly sets up a column chromatography experiment. Although the mixture
is soluble in the mobile phase, the sample appears "stuck" at the top of the column and will
not elute regardless of how much solvent is applied. What could be the cause of this prob-
lem and what could the student do to try and achieve a successful separation?
NA disolves in H₂0
Salt NA
wote H
$22
ер
maybe
e Compound NASI is to much."
anthe solvent being 120
being Alzo is to little v
3. A student performs a separation by TLC on a silica get plate that results in three spots. A
red spot is measured 23 mm away from the original sample spot, a blue spot is 42 mm
away, and a yellow spot is 49 mm away. The distance from the original spot to where the
solvent stopped is 55 mm.
a) Calculate the Re value for each component in the mixture. (Show your work and report
the correct number of significant figures.)
highest polarity. Explain your answer.
b) The silica gel plate is polar. Rank the three components in the mixture from lowest to
d
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Transcribed Image Text:Experiment 2 Chromatography 2. A student properly sets up a column chromatography experiment. Although the mixture is soluble in the mobile phase, the sample appears "stuck" at the top of the column and will not elute regardless of how much solvent is applied. What could be the cause of this prob- lem and what could the student do to try and achieve a successful separation? NA disolves in H₂0 Salt NA wote H $22 ер maybe e Compound NASI is to much." anthe solvent being 120 being Alzo is to little v 3. A student performs a separation by TLC on a silica get plate that results in three spots. A red spot is measured 23 mm away from the original sample spot, a blue spot is 42 mm away, and a yellow spot is 49 mm away. The distance from the original spot to where the solvent stopped is 55 mm. a) Calculate the Re value for each component in the mixture. (Show your work and report the correct number of significant figures.) highest polarity. Explain your answer. b) The silica gel plate is polar. Rank the three components in the mixture from lowest to d
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