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Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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RNA was isolated from a tissue with a solution buffered at pH 12.  However, no RNA was obtained. Why?

a.

 

The 5’ OH group of the ribose is protonated (positive charged) at pH 12 and reacts with the phosphorus atom to cleave the phosphodiester bond.


 

b.

 

The 5’ OH group of the ribose is deprotonated (negative charged) at pH 12 and reacts with the phosphorus atom to cleave the phosphodiester bond.


 

c.

 

The 2’ OH group of the ribose is deprotonated (negative charged) at pH 12 and reacts with the phosphorus atom to cleave the phosphodiester bond.


 

d.

 

The 2’ OH group of the ribose is protonated (positive charged) at pH 12 and reacts with the phosphorus atom to cleave the phosphodiester bond.

 

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