Questions: Which samples can have their own (innate) fluorescence? Part of the spectrophotometer that transmits the desired wavelength: a.monochrome b.Selector c.Detector d.prism
Q: Visit www.mayoclinic.org to learn more about the gallbladder's role in fat breakdown. What dietary…
A: The dietary suggestions following gallbladder removal. 1. For at least a week following surgery,…
Q: Discuss physiology of protozoan
A: Protozoans are the unicellular eukaryotic organisms that can be considered as free living or…
Q: H B H H A H H What do we call the bonds labelled A and B? O Covalent bond, hydrogen bond O Hydrogen…
A: Water is a polar in nature and made up of 2 hydrogen and 1 oxygen molecule , water is used in…
Q: A) B) F A moose eats a lily pad. Consider the model above. The lily pad produces materials (D) used…
A: ANSWER) In order to answer this question we need to understand the concept of photosynthesis, The…
Q: Discuss the permeability of the phospholipid bilayer to the molecules and ions listed below. Is the…
A: The phospholipid bilayer from the plasma membrane or any biological membrane in a cell. The plasma…
Q: Assume that the probability that any child born in the population will be a boy is 0.51 and the…
A: Introduction:- Probability is a number that reflects the chance or likelihood that a particular…
Q: Create a Venn diagram comparing and contrasting the anatomy and physiology of the respiratory system…
A: Introduction: Respiratory system is the network of organs and tissues that help us to breath. It…
Q: a model that represents action potential and synapses. must explain why
A: Action potential or impulse refers to an electrical or chemical signal that is transmitted along the…
Q: explain the concept of coevolution?
A: Introduction: The process of evolution involves constant change and includes trends like rising…
Q: Orte en In How can pressure, size of the molecule, and viscosity affect the rate of diffusion,…
A: Diffusion can be defined as a movement of molecules from the region of their higher concentration to…
Q: You have decided to design a CSTR with recycle to degrade TCE (trichloroethylene) cometabolically by…
A: Introduction :- Small, single-celled organisms called bacteria exist. Nearly all areas of the world…
Q: Question 23 Which process is not an example of post-transcriptional control of gene expression:…
A: Introduction :- Gene expression is the process through which information from a gene is utilised in…
Q: What is the average number of alleles for Population 2? Population 1 Population 2…
A: A gene, a heritable component that regulates a particular characteristic of an organism, is known as…
Q: Observations in Visceral Muscle Observations in Skeletal Muscle Observations in Cardiac Muscle
A: Muscles are tissues that are present around the internal organs. They protect these delicate organs…
Q: Selection confers a reproductive advantage to individuals based on their adaptations, and therefore…
A: When reproduction takes place, the two types of gametes are coming together and form a zygote which…
Q: Describe the process of meiosis
A: Introduction: Mitosis and meiosis are the two distinct processes of cell division. The cell division…
Q: Using the photosynthesis model, consider each of the statements. Choose ALL of the statemer describe…
A: Photosynthesis is a photochemical process (it requires light for the reaction) by which…
Q: Why will a strict anaerobe grow in thioglycollate broth even when the medium is exposed to…
A: Introduction :- Anaerobic bacteria are microorganisms that can thrive without oxygen. For instance,…
Q: Discuss two direct methods in determining microbial population. Discuss it comprehensivel
A: Microbes are the organisms which are very small in size,usually microscope is required to observe…
Q: Explain the events that would lead to uniparental diploidy in an organism
A: A mutation in genetics is an adjustment to the nucleic acid sequence of an organism's, virus's, or…
Q: _____ is the combination of a seat, elation, another modifications to the histones that allow for…
A: Epigenetics is the study of stable phenotypic changes which do not involve alterations in DNA…
Q: what kind of chemicals might you add to try to speed up in the action of an enzyme or to inhibit its…
A: Enzymes are very crucial for chemical reactions as they act on the reactants/substrate in such a way…
Q: Place the following steps of DNA replication in order: Origin melting RNA priming Helicase loading…
A: DNA replication is the process by which the genetic material of a cell is duplicated before the cell…
Q: You have just started a rigorous exercise. Explain two different ways you can increase blood flow to…
A: Everyone requires physical activity and should be permitted to participate in their chosen…
Q: Consider the steps involved during the initiation of replication, explain what will happen if one of…
A: Introduction :- One DNA molecule replicates into two daughter molecules through a process known as…
Q: Homozyogous wild type male Drosophila PPQQRRSSTTUUVV (all linked) are irradiated to induce the…
A: Genes are small units of heredity, a different version of the same gene is known as its allele.…
Q: You have been put in charge of managing a population of the endangered pink fairy armadillo. The…
A: We know that Nt = No*2ñ Nt is population after time t No is initial population n is time taken…
Q: Expansion microscopy technique beats the 0.2 um resolution limit of optical microscopy by improving…
A: Microscopes are instruments that allow enlarged visualization of biological specimens. They work on…
Q: True/False: Mature mRNA are exported out of the nucleus only if they have a 5' cap and poly A tail.…
A: Introduction :- In genomics, a nucleus is the membrane-enclosed organelle within a cell that holds…
Q: True/False: The small subunit of a ribosome catalyzes the formation of the peptide bonds that link…
A: Introduction: An mRNA molecule is attached to by a little ribosomal subunit during translation. A…
Q: Problem 5: The following is a complex pedigree for two traits, red hair and body hair. What are the…
A: a. The genetic basis of these trait is that red hair is autosomal recessive trait and red beard and…
Q: Of what value is Gram staining?
A: Staining the bacteria can be (a) Simple staining that reacts uniformly to all cell types and only…
Q: you know that 1.1OU=1um. you have an organism that measures at 12 O.U at 10x. How long is your…
A: The capacity of a microscope to create an image of an object at a scale bigger (or even smaller)…
Q: List three common components of microtubules and microfilaments.
A: Microtubules are arranged in the form of microtubule organizing centers. They are structures found…
Q: List and describe the stages of the cell cycle.
A: "Cell division" is a set of processes that occur in a cell leading to its maturation and eventual…
Q: Explain translocation, deletions and duplications
A: Chromosome is organized structure of DNA and proteins present in the nucleus of eukaryotic cell.Each…
Q: 1. Cardiomyocytes contractile rate increases as Ca2+ levels in cytosol rise. Releasing of Ca²+ to…
A: Cells can respond to environmental signals via signal transduction pathways. A signal is amplified…
Q: Gene silencing is mediated by a number of short (~20 nt) noncoding RNAs known as what? - These…
A: Gene silencing It controls gene expression to determine cell fate and also controls metabolism and…
Q: 20. Record the type of test used to test for the following organic compounds and what result…
A: To determine whether organic chemicals are present, straightforward chemical tests can be performed…
Q: Which micronutrient can be destroyed by chelating agents? O a. selenium Ob.phosphorous Oc. iron…
A: An organic chemical complex known as a chelate is one in which the metal component of the molecule…
Q: What do you understand by “Sequencing by synthesis?” What is FRED score? Give an example.
A: Introduction: The complete DNA sequence of a human being is known as their genome. There are 46…
Q: Complete Table I to summarize the function of organelles found in eukaryotic cells. Table 1 The…
A: In the question, it is asked to summarize the function of organelles in the eukaryotic cell.…
Q: when it rains why do we see more worms out? Use the scientific method steps to describe why we see…
A: Earthworms are a sign of good soil. These are necessary for the breakdown of organic debris (such as…
Q: hunter fisher gatherers: Explain the methods of food production by these peoples/societies
A: The phrase "hunter-gatherers-fishers" is now frequently used to describe people who rely heavily on…
Q: Activity: Who's the Real Father? Nathaniel lost his mom Rachel. According to the family lawyer, all…
A: There are mainly four blood groups A, B, Ab and O. These blood groups are named according to the…
Q: ( 176 > >>> 17% 119 min 18 secs Compare the features of RNA polymerase III and DNA polymerase III in…
A: Introduction: Only one DNA strand may code for one strand of RNA during transcription. The synthesis…
Q: how do unicellular differ from single cell multicellular organisms differ
A: Organisms are categorized into different types such as I) on the basis of nuclear membrane/nucleus…
Q: 1. What 3 different ways ATP is used in skeletal muscle contraction? 2. What role is played by…
A: A muscle contraction (also known as a muscle twitch or simply twitch) occurs when a muscle cell…
Q: Compare and contrast between mitosis telophase and meiosis telophase I
A: Mitosis and meiosis are types of cell division that are seen in both plants and animals. Mitosis is…
Q: Hos is the contractile vacuole different in appearance from the Amoeba
A: A contractile vacuole is a kind of vacuole that expands to gather water and its accompanying solutes…
Questions:
Which samples can have their own (innate) fluorescence?
Part of the spectrophotometer that transmits the desired wavelength: a.monochrome b.Selector c.Detector d.prism
Step by step
Solved in 2 steps
- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawSelect ALL the correctly matched terms: a. Immersion oil/ Oil with a different refrative index than air, used to enhance magnification b. Luminescence/ Absorption of light and energy within a sample c. Fluorescence/ Absorbed light rays are changed into longer wavelengths and re-emitted d. Diffraction/ Bending of light rays around an openingHow can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!
- Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 µm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…Objective lens A has a limit of resolution equal to 0.2 mm while objective lens B has 30 μm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?
- a. The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes in absorbance? b. Convert the 98%T to absorbanceThe significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.The following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next? After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?
- Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?Question 1: Describe what you see through the microscope as compared to how the word "roe" looked when you placed the slide on the stage. a) Describe what happened to the image as the total magnification was increased. In other words, how much of the specimen (the word roe) is visible at each level of magnification, 4x, 10x, and 40x? Question 2: Given the images you have seen, please consider how it would be to need to find the red and white blood cells on this slide. List at least 3 observations for the blood slide. When discussing a specific image, make sure to note the Total Magnificiation (TM). a) List another three observations below based on the bacterial slide images. Note the Total Magnification used when comparing blood and bacterial slides.