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- Choose for the organisms below, depending on the incubation condition and media whether they will grow or not 1.Haemophilus influenzae in HBA CO2 2.Haemophilus influenzae in HBA anaerobic 3.Haemophilus influenzae in Chocolate agar CO2 4.Haemophilus influenzae in MacConkey 5.Clostridium perfringens in HBA CO2 6.Clostridium perfringens in HBA anaerobic 7.Clostridium perfringens in Chocolate agar CO2 8.Clostridium perfringens in MacConkey 9.Escherichia coli in HBA CO2 10.Escherichia coli in Chocolate agar CO2 11.Escherichia coli in MacConkey 12.Escherichia coli in HBA anaerobic.3- Visible Growth in Tubes Growth in Petri Dishes 0 µg/ml 2 µg/ml 0 1 3 4 ÖÖÖÖÖÖÖÖ 2 5 6 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information; a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c.…1.Differentiate between a microaerophile and an aerotolerant organism. 2.Why is resazurin a useful media additive for the study of anaerobes? 3.Why is a GasPak anaerobic jar necessary for the culture of anaerobes on plates of Brewer's anaerobic agar but not in tubes of fluid thioglycollate medium (FTM)? 4.Where in the human body would you expect to find an obligate aerobe? Where would you expect to find an obligate anaerobe?
- Home Tools 6weekwomensfull... ↓↓ Nelson Biology 11... 8.27 x 11.69 i 3 / 7 Unit 1- Quiz on Le... x 188% 7. Which of the follow is false with regards to Eubacteria? These bacteria can engage in either [or both] aerobic and anaerobic respiration O These bacteria form endospores to reproduce There are three types of these bacteria; thermophiles, methanogens, halophiles Both B and C are false Vimin that ramain daumont within the call munt ha in thin /thann svalafal 1 p Sign In (S C E ETOPIC : MICROBIAL GROWTH Classify the bacteria that is most likely involved in each situation provided according to their classification (as to microbial growth requirements). CHOICES: A. Obligate aerobe ,B. Obligate anaerobe, C. Microaerophile, D. Aerotolerant anaerobe, E. Mesophile, F. Thermophiles 1.The bacteria under study thrives at the alveolar ducts in the lungs causing respiratory disease. 2.In the experiment, the agar plate containing an axenic culture was originally incubated inside a candle jar for one day and then transferred to an oxygen-rich incubator for another day. Findings: the microbial growth persisted and stayed the same regardless of the specific type of incubation employed. 3.A microorganism that can survive pickling and refrigeration was discovered when a tightly-sealed jar of pickles grew several colonies despite following the correct recipe, tight closure seal, and proper refrigeration. 4.The spoiled bottle of wine that was stored at standard room temperature…1. Why does a precipitate form on Sierra's medium when incoulated with a lipolytic organism? Give the reasons and explain it thoroughly.
- Temperature plays a very important role in the growth of fungi, bacteria, and other microorganisms. Most bacteria grow at optimal temperatures. Mesophiles are grown mainly at optimum temperature. What is is the impact of the above on a) on unpasterized milk? 2. Expired date and off-putting odor?Anglais Question 9. With three examples, explain how co- culture could contribute to the growth of uncultivable bacteria?Alumno.l 4:07 p. m 56 % UNIT 2 REVIEW Chapter 6: 1. Compare the resolution of the light microscope with that of the electron microscope 2. What part of the cell is best yisualized with a scanning electron microscope? 3. What part of the cell is best yisualized with a transmission electron microscope? 4. Explain the process of cell fractionation 5. Which organelle is isolated at the slowest speed on a centrifuge? Which organelle is isolated last after stepwise increase in centrifuge speed? 6. What organelles do prokaryotic and eukaryotic cells have in common? 7. Know the location and function of the following organelles: nuclear envelope, endoplasmic reticulum, Golgi Complex. lysosome, vesicles, vacuoles mitochondria, chloroplasts. peroxisome, centrioles/centrosome, cilia/flagella. cytoskeletal elements, cell wall and extracellular matrix 8. Know the four types of intercellular junctions and what function they have in cells Chapter 7: 1. Describe the molecules contained within the cell…
- 1. What is the name of the cultures used? 2. Where they gram negative or positive? 3. Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.Explain 2 factors that influence microbial growth.a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?