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- QUESTION NUMBER: 23 Transcription of the zygote nucleus is dispensable in Drosophila early development but not in early development of mouse. Why? Pick the correct statement in this context: A) O The above assumption is wrong, transcription of the zygote nucleus is essential for Drosophila as well as mouse early development. B) O Elaborate epigenetic mechanisms present in mouse, but absent in Drosophila pose an essential need for the zygote nucleus to be transcriptionally inactive in mouse. C) O Drosophila oocyte contains all the transcripts needed to support early development, but the mouse oocyte does not. D) O Due to inactivation of one X chromosome in early development, mouse oocytes have developed independent regulatory strategies to dispense with the early zygoticQuestion 4: Explain how a deletion mutation leading to a modification of the 3' untranslated region (UTR) on the CACNA1S mRNA could lead to hypokalemic periodic paralysis without changing the structure of the polypeptide formed? (Note this is not the known cause of hypoKPP, but it makes a good question)Question:- Transformation of host cells by several DNA viruses requires inactivation of the cellular proteins pRB, CKI ( cyclin-dependent kinase inhibitor) and p53. give an example of how each of these cellular proteins can be inactivated by DNA viruses. ( one example/cellular protein)
- What is cell differentiation? Discuss the role of myogenic bHLH proteins in the differentiation of muscle cells. Explain how they work at the molecular level. In your answer, explain how protein dimerization is key to gene regulation.QUESTION 5 Which of these statements best describes the major difference between enhancers and promoter proximal elements (PPES)? Enhancers activate transcription; PPES inhibit transcription Enhancers are located far away from the core promoter; PPES are close to the core promoter Enhancers are long regions of DNA; PPES are short RNA molecules Enhancers are transcription factors; PPES are DNA sequences O O O OQuestion: how does the flu without the track arise and what does it mean male type splicing if the flu protein isn't present in females?
- Which statements are true? Explain why or why not.1 In terms of the way it interacts with DNA, thehelix–loop–helix motif is more closely related to the leu-cine zipper motif than it is to the helix–turn–helix motif.2 Once cells have differentiated to their final spe-cialized forms, they never again alter expression of theirgenes.3 CG islands are thought to have arisen during evo-lution because they were associated with portions of thegenome that remained unmethylated in the germ line.4 In most differentiated tissues, daughter cells retaina memory of gene expression patterns that were presentin the parent cell through mechanisms that do not involvechanges in the sequence of their genomic DNA.Question for assignment: Using a transgenic technique, propose an experiment to determine whether Cdx2 is sufficient for trophoblast development in the mouse embryo. Describe two results that you would expect to observe at the blastocyst stage if Cdx2 is indeed sufficient for trophoblast development. Be as specific as possible regarding the transgene that you propose for this experiment (including what gene's enhancer you would use in the transgene). Note: you do not need to explain the details of how a transgenic mouse is made. Describe the experiment in steps (Step 1: ..., Step 2: ... etc) and please keep your answer to under 150 words. TIPS FOR ANSWERING: DONT talk about stop cassetes/memory cassetes, focus on transgenes Paper (below) gave lots of results that you might see,, 6 diff ways that cdx2 is required for trophoblasts need specific gene enhancer (dont just say "expressed enhancer in genital ridge")Question for assignment: Using a transgenic technique, propose an experiment to determine whether Cdx2 is sufficient for trophoblast development in the mouse embryo. Describe two results that you would expect to observe at the blastocyst stage if Cdx2 is indeed sufficient for trophoblast development. Be as specific as possible regarding the transgene that you propose for this experiment (including what gene's enhancer you would use in the transgene). Note: you do not need to explain the details of how a transgenic mouse is made. Describe the experiment in steps (Step 1: ..., Step 2: ... etc) and please keep your answer to under 150 words. TIPS FOR ANSWERING: DONT talk about stop cassetes/memory cassetes, focus on transgenes Paper (Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocust) gave lots of results that you might see,, 6 diff ways that cdx2 is required for trophoblasts need specific gene enhancer (dont just say…
- Based on standard MS- LS3-1: Fish in a cave system in Mexico is missing its eyes, has thin, translucent skin, and is relatively small (7-10 cm in length). Can you describe by model why structural changes to genes (mutations) on chromosomes may affect proteins and may result in beneficial effects to the structure and function of the fish? Can you answer in the following format? 1- Structure How Structure and Function is Affected by Mutations in Blind Fish Eyes Scales Taste Cells Lateral Line 2- Model to explain what causes these changes: Change: ______________ Adapting an Organism to the Dark Cause: ________________ Stop the Growth of Eyes Effect: ___________________ Fish with Heightened Other Senses References: Video: Rare Blind Cave Fish in Mexican Cave System https://www.youtube.com/watch?v=MWdtGuDd8z0 Fact Sheet: Blind Cave Fish https://www.denverzoo.org/animals/blind-cave-fish Information: Mexican Tetra…Time (h) 0 1 3 6 12 Mouse thymus cells were treated with 10 micromolar dexamethasone to induce apoptosis. At the indicated times the chromosomal DNA was extracted and analyzed by gel electrophoresis, which separates the DNA fragments based upon size differences. When cells undergo apoptosis their DNA is cut into fragments that are approximately multiples of 200 base pairs. This results in a characteristic “ladder" appearance when the fragments are separated by gel electrophoresis, as shown above. Based upon what you know about eukaryotic chromatin, explain why this pattern of DNA fragments is obtained. 6.BL1250 Group activity 4 2020 (1) – Saved to my Mac v Mailings Review View Tell me EXERCISE 4 QUESTIONS 1. TRANSCRIPTION: Much research effort has been invested in understanding how transcription is regulated, because this process is very important for determining cell function. Transcription is a major on/ off switch for genes, providing the instructions for translation and determining the types and amounts of proteins made in a cell at a specific time. The Zebrafish is a model organism for research, and many biologists study how the regulation of genes affects the development of the zebrafish embryo. The effects of a variety of experimental conditions on zebrafish embryos can be easily visualized by light microscopy, because the outer covering (chorion) and the embryo are both transparent. See an images of zebrafish embryos at different time points in development and the adult form below. 0h Os 0.75 h 2h 3.25 h 8 h 16 h 24 h 72 h Adult -3 months (not to scale) Image from:…