Question:- In an early log-phase E. coli population, there are 1 x 103 cells/ml the generation time of the cells is 30 minutes. How many cells would there be 60 minutes later? a. 2X10^6 b. 8X10^3 c. 4X10^3 d. 2X10^3 e. 3X10^6
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Question:-
In an early log-phase E. coli population, there are 1 x 103 cells/ml the generation time of the cells is 30 minutes. How many cells would there be 60 minutes later?
a. 2X10^6
b. 8X10^3
c. 4X10^3
d. 2X10^3
e. 3X10^6
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- D Question 9 An enzyme assay of 2 mL of extract shows 7.3 U/mL and a biuret assay shows 4 mg/mL protein on the initial sample. It is salted out and the pellet is washed and resuspended in 0.4 mL buffer. It is re-assayed and shows 12.1 U/mL activity and 1.3 mg/mL protein. What is the change in fold purity for this sample? (answer to 2 decimal places)Question 3 Listen Which of the following statements describe disadvantages of the classical Sanger sequencing approach when compared to the modern Sanger sequencing method? |Radioactivity must be used, representing an added hazard Fluorescence is used, representing an added hazard Four reaction are used, requiring more reagents and escalating cost OGel electrophoresis is used to separate the various products by size but slowing overall completion of sequencing OA single reaction is used, complicating the analysis O Capillary electrophoresis is used to separate the various products by size but slowing overall completion of sequencing Question 4 4 Listen Electrophoresis of polynucleotides can be done using either agarose or polyacrylamide gels. What is the primary consideration when choosing which polymer gel to use? O Overall charge on polynucleotide sample, greater overall negative charge requires agarose gel with smaller pores to slow the migration of the highly charged sample O…Question 3 Listen Imagine that you took a stock of 1,000,000 pfu/mL of bacteriophage and made a series of dilutions by taking 1mL from the stock and adding it to 9mL of fresh broth. You then mixed that tube and repeated 2 more times, each time taking 1mL and adding it to 9mL of fresh broth, for a total of 3 dilutions. THEN you added a few drops of E. coli and plated 1mL from your final tube on a TSA plate. What would you observe after 24 hours incubation? It might help to draw a picture. (How many plaques?) 1 10 100 1,000 10,000 100,000 1,000,000
- Remaining Time: 1 hour, 26 minutes, 16 seconds. Question Completion Status: A Moving to another question will save this response. Question 10 In which type of transfer do bacteria or archaea take up DNA from their environment and integrate it into their genome? O a. transduction O b. vertical O C. transformation O d. conjugation O e. all horizontal A Moving to another question will save this response. HUAWEI F7 F8 F1 F2 F3 F4 F5 F6 17Question:- What must two different bacteria have in common for the same bacteriophage to be able to kill both types?Question 15 How did you select and grow a streptomycin resistant strain of bacteria in this experiment? 1. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin negative plate. Colonies that grew carried a mutation for resistance 2. A colony of bacteria was with streptomycin so that the antibiotic could alter then genetic composition of the bacteria . 3. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin positive plate. Colonies that grew carried a mutation for resistance 4. Samples of bacteria were taken from the culture and observed under a microscope for signs of susceptibility or resistance . Those that were resistant were separated and plated .
- Question 15 How did you select and grow a streptomycin resistant strain of bacteria in this experiment? 1. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin negative plate. Colonies that grew carried a mutation for resistance 2. A colony of bacteria was with streptomycin so that the antibiotic could alter then genetic composition of the bacteria . 3. A sample of bacteria was exposed to streptomycin by inoculating it onto a streptomycin positive plate. Colonies that grew carried a mutation for resistanceReport the slope and the y-intercept below. Slope () = = y-intercept () = Can this standard curve be used for E.coli cells (why/why not?)Question 9 What is the most likely reason that there were so few bacterial colonies growing on the streptomycin-positive plate in the first part of the experiment? 1. Streptomycin in the medium caused deleterious mutations in the bacteria. 2. Only a few individuals in the original bacterial culture had the mutation that conferred resistance to streptomycin .
- https://studylib.net/doc/8245959/lab-7--got-milk%3F follow and open the link then answer the question number 4: Bacteria grown solely on lactose will grow, as will bacteria grow only on ONPG. However, cells grown solely on TMG will not grow, and will instead eventually die. Based on the structures provided below, why are cells capable of growing on ONPG and lactose, but not TMG alone?please explain this question to me. Also, even though we get 96 in the second option, why is it wrong? help me pleaseQuestion 1: A. In Northern blotting, electrophoresis is used to resolve which biological molecules? What type of probe is used to identify the target molecule(s)? B. In Southern blotting, electrophoresis is used to resolve which biological molecules? What type of probe is used to identify the target molecule(s)? C. In Western blotting, electrophoresis is used to resolve which biological molecules? What type of probe is used to identify the target molecule(s)? D. Which of these techniques gives information about the expression levels of genes?