Question 24 Which of the following are biolegical functions of the citric acid cycle? O Completing the reduction of carbon trom glucose to CO2 OPraduction of GTP which can be interconverted with ATP Production of vibese 5 phosphate Generation of FADH2

Question 7 The uncoupling agent 2,4-dinitrophenol (DNP) has a pką of about 7.2. Which of the following describes how this molecule works to uncouple the proton gradient? NO2 O2N- 2,4-Dinitrophenol (DNP) DNP is initially protonated in the intermembrane space, then deprotonated after crossing to the matrix, thus dissipating the proton gradient. DNP is initially deprotonated in the intermembrane space, then protonated after crossing to the matrix, thus dissipating the proton gradient. O c) None of the answers is correct. DNP is initially deprotonated in the matrix, then protonated after crossing to the intermembrane space matrix, thus dissipating the proton gradient. DNP is initially protonated in the matrix, then deprotonated after crossing to the intermembrane space matrix, thus dissipating the proton gradient.

QUESTION 35 During aerobic cellular respiration, energy travels downhill in which sequence? o Food molecules → ATP → electron transport chain → oxygen O Food molecules → NADHH electrochemical gradient → ATP o Food molecules → pyruvate → ATP oxygen O Food molecules → citric acid cycle → ATP→ NAD* o Food molecules → glycolysis → FADH2 → 02 ATP

QUESTION 1 As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light- driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the bacteriorhodopsin pumps H+ in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine light onto the suspension of vesicles. What do you observe? purified ATP synthase purified bacteriorhodopsin detergent ADD PHOSPHOLIPIDS AND REMOVE DETERGENT LIGHT sealed vesicle (liposome) Figure Q14-18 Essential Cell Biology ale te Garland Science 2010) A NO ATP synthesis since no electron transport occurs. B. ATP synthase makes ATP. CATP synthase hydrolyzes ATP to pump H+ into the vesicles. D.No ATP synthesis since no H+ gradient is created. Click Save and Submit to save and submit. Click Save All Answers to…