Question #1: In 1966, a mouse was found in the Jackson Laboratory colony that seemed to be excessive in weight as compared to its wildtype siblings from the same litter. The allele that was assigned to this mouse was called "db". Given the data from the original 1966 paper below, a) is there evidence of a genetic basis for an increased overall weight of the mutant mouse? b) Is this mutant phenotype encoded by a dominant or recessive gene? Please provide an explanation of your answers. Table 1. Body weight of diabetic (dbdb) and normal (++ and + db) mice. Sexes are combined. Age (weeks) 2- 3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 11-12 12-13 13-22 23-54 No. of mice 6 12 25 20 16 12 10 11 11 11 7 5 11 5 dbdb Av. 6.7 14.8 19.8 25.4 28.3 32.4 34.5 34.3 37.9 39,4 38.1 44.4 45.3 45.9 Weight (g) Range 7- 10 9- 20 10-27 AGIGTAAAC 14- 34 18-39 21-42 31-41 31-44 32- 45 32-46 34- 51 32-62 37-61 last two nucleotides of the 106 bp No. of mice 7 21 16 19 17 17 5 26 ++and+ db Av. 7.6 11.3 16.9 18.5 19.8 21.9 27.3 28.9 Weight (g) Range 7-9 9-13 In 1996, the genetic basis of this mutation was mapped to the leptin gene and no large chromosomal variations were found in the db variant or around the leptin gene as compared to wildtype siblings. c) What was the likely mechanism by which this mutation arose in the mutant mouse? .exon...AG|GTAAGT...intron... 43 882843 76686 15- 21 15- 23 16- 28 19- 28 Also in 1996, the genetic basis of the change was found to be a mutation in the splice site of the exon, with a single mutation of G to T. d) What was the likely lab technique used to identify this nucleotide change? AG GGAAAC 20-36 20-38 wt/wt db/db consensus
Question #1: In 1966, a mouse was found in the Jackson Laboratory colony that seemed to be excessive in weight as compared to its wildtype siblings from the same litter. The allele that was assigned to this mouse was called "db". Given the data from the original 1966 paper below, a) is there evidence of a genetic basis for an increased overall weight of the mutant mouse? b) Is this mutant phenotype encoded by a dominant or recessive gene? Please provide an explanation of your answers. Table 1. Body weight of diabetic (dbdb) and normal (++ and + db) mice. Sexes are combined. Age (weeks) 2- 3 3-4 4-5 5-6 6-7 7-8 8-9 9-10 10-11 11-12 12-13 13-22 23-54 No. of mice 6 12 25 20 16 12 10 11 11 11 7 5 11 5 dbdb Av. 6.7 14.8 19.8 25.4 28.3 32.4 34.5 34.3 37.9 39,4 38.1 44.4 45.3 45.9 Weight (g) Range 7- 10 9- 20 10-27 AGIGTAAAC 14- 34 18-39 21-42 31-41 31-44 32- 45 32-46 34- 51 32-62 37-61 last two nucleotides of the 106 bp No. of mice 7 21 16 19 17 17 5 26 ++and+ db Av. 7.6 11.3 16.9 18.5 19.8 21.9 27.3 28.9 Weight (g) Range 7-9 9-13 In 1996, the genetic basis of this mutation was mapped to the leptin gene and no large chromosomal variations were found in the db variant or around the leptin gene as compared to wildtype siblings. c) What was the likely mechanism by which this mutation arose in the mutant mouse? .exon...AG|GTAAGT...intron... 43 882843 76686 15- 21 15- 23 16- 28 19- 28 Also in 1996, the genetic basis of the change was found to be a mutation in the splice site of the exon, with a single mutation of G to T. d) What was the likely lab technique used to identify this nucleotide change? AG GGAAAC 20-36 20-38 wt/wt db/db consensus
Human Anatomy & Physiology (11th Edition)
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Transcribed Image Text:Question #1: In 1966, a mouse was found in the Jackson Laboratory colony that seemed to be
excessive in weight as compared to its wildtype siblings from the same litter. The allele that was
assigned to this mouse was called "db".
Given the data from the original 1966 paper below, a) is there evidence of a genetic basis for
an increased overall weight of the mutant mouse? b) Is this mutant phenotype encoded
by a dominant or recessive gene? Please provide an explanation of your answers.
Table 1. Body weight of diabetic (dbdb) and normal (++ and + db) mice. Sexes are
combined.
Age
(weeks)
2- 3
3-4
4-5
5-6
6-7
7-8
8-9
9-10
10-11
11-12
12-13
13-22
23-54
No.
of
mice
6
12
25
20
16
12
10
11
11
7
5
11
5
dbdb
Av.
6.7
14.8
19.8
25.4
28.3
32.4
34.5
37.9
39,4
38.1
44.4
45.3
45.9
Weight (g)
Range
AG|GŽAAAC
7- 10
9- 20
10-27
14- 34
18-39
21-42
31-41
31-44
32- 45
last two nucleotides of the 106 bp
32-46
34-51
32- 62
37-61
No.
of
mice
7
21
16
19
17
17
5
26
++and+ db
Av.
7.6
11.3
16.9
18.5
19.8
21.9
Weight (g)
Range
7- 9
9- 13
15- 21
15- 23
16- 28
19- 28
In 1996, the genetic basis of this mutation was mapped to the leptin gene and no large
chromosomal variations were found in the db variant or around the leptin gene as compared to
wildtype siblings. c) What was the likely mechanism by which this mutation arose in the
mutant mouse?
20-36
20-38
Also in 1996, the genetic basis of the change was found to be a mutation in the splice site of the
exon, with a single mutation of G to T. d) What was the likely lab technique used to identify
this nucleotide change?
AG GGAAAC
wt/wt
db/db
.exon...AG GTAAGT...intron... consensus
Transcribed Image Text:last two nucleotides of the 106 bp.
AGIGTAAAC
...exon. .AG GTAAGT...intron... consensus
Using the following gel electrophoresis image of amplified leptin mRNA, e) what was the
impact of the altered splice site (db mutation) on leptin mRNA? db-homozygous mice,
c=homozygous wildtype mice
1.6-
1.0-
0.5-
Hypo
db/db
Brain
kb M db C db C db C db C
Liver
0 36 15 21 28 Days
Lung
db C
The expression of the leptin gene was then further explored in knockout mice (db/db) and
wildtype mice (+/+).
f) Given these RNA expression results, what would you expect the Western blot to look
like in each type of mouse? Please sketch the blot and explain it in your own words
db/db
036 Days
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