Q: Antibiotics are used in genetic engineering.They are usefulto keep culture free of microbial aOinfectionsOto select healthy vectors .bOto identify replication start sites.cOas selectable markers .dQ: Choose the best choice that describes thepurpose of adding chloramphenicol to theculture of engineered bacteriaOIt inhabits the process of protein asynthesisOIt blocks chromosome replication .bIt blocks cell division .cIt leads to increase the copy number of drecombinant moleculesQ: Alkaline denaturation process is a kind ofDNA isolation on the basis of conformation, thatcause insolubility of cell components except oneof the followingSupercoiled DNA aChromosomal DNA bProtein molecules cRNA molecules dAll choices.eNone of choices fQ: After transforming the ligation mixture intothe E. coli, the transformed and non-transformed bacteria both can grow on media.containing antibioticsTrue aOFalse ..b

ANS. 1 OPTION D- In genetic engineering the vectors have ANTIBIOTIC RESISTANT GENE. This gene helps…

Answered: Q: Antibiotics are used in genetic…

Phlebotomy Essentials

6th Edition

ISBN:9781451194524

Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Chapter1: Phlebotomy: Past And Present And The Healthcare Setting

Section: Chapter Questions

Problem 1SRQ

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Match each characteristic to the proper bacterial fertility factor type. F bacterium F+ bacterium F bacterium Hfr bacterium Answer Bank recipient of DNA during bacterial conjugation bacteria with F factor integrated into genome contains a plasmid with only the F factor does not contain the F factor sequences contains a plasmid with the F factor and some genomic DNA
In terms of microbial pathogenicity, molecular Koch's postulates are a set of experimental criteria that show: A microbe is the aetiological agent of a disease A gene encodes a product that contributes to disease A gene encodes a product that contributes to immunity to a disease A mutation does not contribute to disease
Classify each statement as true of generalized transduction or specialized transduction. Generalized transduction Specialized transduction Answer Bank Genes transfer from random sites in the bacterial chromosome. This utilizes lysogenic phages. Genes that transfer depend on the prophage insertion site. This utilizes lytic phages. Phage inserts itself at a specific site in the bacterial chromosome. This requires accidental packaging of bacterial DNA in viral capsids.
About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.
Row C D. B B. one: A C O phage with radiolabelled protein coat phage with radiolabelled DNA 100 10. phage infects The experiment shown above was designed by bacterium phage infects bacterium EXPERIMENT 1 phage shell is removed EXPERIMENT 2 요 Hershey & Hershey & Chase Meselson & Stahl Meselson & Stahl 8 phage shell is removed 28 no radioactivity in cells ii 48 radioactivity in cells LL and proved that ii DNA replication is semiconservative DNA is the hereditary material DNA replication is semiconservative DNA is the hereditary material (select the row that correctly completes the statement)
Which of the following does NOT describe a mechanism for drug resistance? Prevention of drug entry into the cell Production of proteins that bind and sequester the drug Creation of new plasmids upon drug treatment Changes in the structure of the drug's target Inactivation of the drug by hydrolysis
QUICK I WILL RATE NO EXPLANATION NEEDE D Which of the following is not correct regarding genetic material transfer? When virulence plasmids are transferred to the bacteria, they become virulent The resistance plasmids also carry transposons and IS elements that have resistance genes to antibiotics IS elements and transposons can cause mutations in genes Insertion sequences and transposons are fixed genetic elements of chromosomal origin
phage can be "induced" as a result of DNA damage. Consequences of induction include. Temperate phage, e.g lambda phage; lytic cycle and transduction Lytic T4 phage, assembly, conjugation O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation Temperate phage, e.g. lambada phage, prophage formation, transformation O T4 phage, lytic cycle, specialized trasnduction
Understanding the life cycle of Plasmodium is important and we know that it is spread via mosquitos. The mosquito serves as O a mechanical vector in which the pathogen does not need the vector for replication O a biological vector in which the pathogen does not need the vector for replication O a mechanical vector in which the mosquito serves as a host for the multiplication of the pathogen during some stage of the pathogen's life cycle O a biological vector in which the mosquito serves as a host for the multiplication of the pathogen during some stage of the pathogen's life cycle Question 34 Patients are becoming inherently resistant to malaria resulting from O Sporogenic cells traveling a shorter distance O Climate changes O Genetic deficiency of glucose-6-phosphate dehydrogenase O Decrease in mosquito numbers 12 ....
E. coli Hfr cells Choose an answer below: can transfer genes to F- cells. can form sex pili (F pili). typically do not transfer their entire genome during conjugation. carry the F plasmid in their chromosome. all of the above
If a bacteria cell is F+, that cell must be capable of transduction. contain the fertility factor. be capable of transformation. contain antibiotic resistant plasmids.
Which of the following apply to the development of urogenital tract Candida albicans infections? Disruption of population dynamics allowing the RB form of Chlamydia to cause tissue damage Loss of Lactobacillus populations leading to an increase in the local pH Loss of Gardnerella vaginalis populations leading to a lose of Clue cells Loss of the anaerobic environment that promotes the outgrowth of Trichomonas vaginalis None of the answers apply

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