Discuss the regulation of the tryptophan operon. How does this story change under the two conditions? - There is little trp in the cell, so the cell needs to make enough, operon is fully transcribed. - There is lots of trp in the cell, so the cell can inhibit the operon's expression. Discuss all mechanisms of inhibition.
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- Match each of the following mechanisms with how it can change the levels of gene products--whether on different operons or within the same operon. Different promoters might have different DNA sequences Translation initiation sequences can cause different open reading 1. Differences in mRNA levels BETWEEN operons frames to be translated 2. Differences in protein levels WITHIN an operon differently. The secondary structures of MRNA can differ which can change the rate of degradation of transcripts.One way that regulation is often studied is by examining the effects of mutations. For example, mutations within the parts of the lac operon have been well studied. Which of these mutants would be considered a constitutive mutant (the operon is always on)? a mutation in CAP that prevents the binding of CAMP a mutation in one of the structural genes that prevents the transport of lactose into the cell a mutation that disables the promoter for the operon a mutation that disables the repressorWhat is a specific region in the trp operon that you could mutate so it expresses the trp operon in conditions of excess tryptophan. Why will this mutation cause this? Operon structure: promoter, operater, structural genes promoter - DNA region that is the site of RNA polymerase sigma binding and transcription initiation operator – DNA region that influences gene expression through its interaction with a protein (transcription factor) structural genes – gene whose expression is being regulated; encodes a polypeptide or functional RNA
- Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.Describe how the components of the trp operon system are affected by a mutation in trpC? Why is it that once enviornmental tryptophan is inserted into the cell, only the trp repressor is activated (turned on)?If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
- Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination. c) Review the region that is responsible for trp attenuation during low trp level.Which of the following statements about the trp operon is false? If a mutation in region 2 prevents the formation of the 2 + 3 loop in the 5’UTR transcription levels will increase. The trp operon is an example of negative repressible operon. Formation of the anti-terminator in the 5’UTR of the trp operon occurs when trp levels are low. In the presence of high trp-tRNA, the genes of the trp operon are not expressed. The role of trp is to bind to the repressor protein and activate it.. In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high
- The trp operon is: **(for additional information review the CogBooks Module, answer explained on CogBooks): In the absence of tryptophan, the trp repressor dissociates from the operator, and RNA synthesis proceeds. promoter operator trpE| trpD trpC trpB trpA RNA polymerase repressor When tryptophan is present, the trp repressor binds the operator, and RNA synthesis is blocked. promoter operator trpE trpD trpC trpB trpA RNA polymerase; repressor tryptophan Both negative inducible and positive repressible Positive inducible Negative Repressible O Negative inducibleThe map of the lac operon is shown below. Consider the following examples that include both haploids and partial diploids and explain in each scenario whether the repressor can bind and regulate expression and whether or not the lac operon is expressed. For partial diploids the plasmid is indicated by the F’. I+ O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ I+ Oc Z+ Y+The TRP operon is regulated by two mechanisms, the TrpR regulatory protein and attenuation. Complete following sentences by picking the right choice: 1. Attenuation occurs in the ___________ (promoter, structural genes, 5' UTR, region upstream of the promoter) , which is divided into four regions. It depends on the amount of ___________ (trp, tRNAs, charged trp tRNAs) in the cell, which is the signal for this type of regulation. 2. If the signal is low in the cell, then the ribosome pauses in region _______ (1, 2, 3, or 4) because the sequence includes several ______ (start codons, sotp codons, trp codons, trp molecules, inverted complementary sequences). Regions ________ (1 and 4, 1 and 3, 2 and 3, 2 and 4, 1 and 2, 3 and 4) will form a secondary structure and ______ (tranlsation continues, transcription stops, transcription continues, translation stops) (choose the first process impacted). 3. If the signal is high in the cell, then regions ______ (2 and 3, 2…