Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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**Predicting the Growth/Color Pattern for a Mutant Unable to Activate Both Sugar Operons**

**Introduction:**
In this exercise, you will predict the growth or color pattern expected for a mutant that cannot activate both sugar operons (such as the lac and mal operons).

**Instructions:**
- Table 1: NA plates options—blue, white, or either.
- Table 2: Minimal plates options—growth or none.

**Table 1: Nutrient Agar (NA) Plates**
|                | NA                                                            |
|----------------|--------------------------------------------------------------|
|                | Xgal                           | Xgal + lactose                            | Xgal + IPTG                  |
|                | [ Select ]                     | [ Select ]                                | [ Select ]                   |
 
- **Xgal:** Select the predicted color outcome (blue, white, or either).
- **Xgal + lactose:** Select the predicted color outcome (blue, white, or either).
- **Xgal + IPTG:** Select the predicted color outcome (blue, white, or either).

**Table 2: Minimal Plates**
|                | Minimal                                                   |
|----------------|-----------------------------------------------------------|
|                | glucose               | lactose                            | maltose         |
|                | [ Select ]            | [ Select ]                         | [ Select ]      |

- **Glucose:** Select if growth or no growth is expected.
- **Lactose:** Select if growth or no growth is expected.
- **Maltose:** Select if growth or no growth is expected.

These predictions will help you understand the metabolic pathways and regulatory mechanisms affecting the operons for sugar utilization in bacterial mutants. Make your selections based on your understanding of the operon model and the specific mutation disabling both sugar operon activations.
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Transcribed Image Text:**Predicting the Growth/Color Pattern for a Mutant Unable to Activate Both Sugar Operons** **Introduction:** In this exercise, you will predict the growth or color pattern expected for a mutant that cannot activate both sugar operons (such as the lac and mal operons). **Instructions:** - Table 1: NA plates options—blue, white, or either. - Table 2: Minimal plates options—growth or none. **Table 1: Nutrient Agar (NA) Plates** | | NA | |----------------|--------------------------------------------------------------| | | Xgal | Xgal + lactose | Xgal + IPTG | | | [ Select ] | [ Select ] | [ Select ] | - **Xgal:** Select the predicted color outcome (blue, white, or either). - **Xgal + lactose:** Select the predicted color outcome (blue, white, or either). - **Xgal + IPTG:** Select the predicted color outcome (blue, white, or either). **Table 2: Minimal Plates** | | Minimal | |----------------|-----------------------------------------------------------| | | glucose | lactose | maltose | | | [ Select ] | [ Select ] | [ Select ] | - **Glucose:** Select if growth or no growth is expected. - **Lactose:** Select if growth or no growth is expected. - **Maltose:** Select if growth or no growth is expected. These predictions will help you understand the metabolic pathways and regulatory mechanisms affecting the operons for sugar utilization in bacterial mutants. Make your selections based on your understanding of the operon model and the specific mutation disabling both sugar operon activations.
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