Plot a calibration graph of absorbance vs concentration of Ca2+ and determine the concentration of Ca2+ in the unknown tap and unknown bottle water samples.

Q: Calculate the specific absorbance ratio (SAR), where A620 = 0.166 and A280 = 0.557

A: The specific absorbance ratio (SAR) is calculated as the ratio of the absorbance at 620 nm to the…

Q: What cause the spikes in the spectrophotometer reading? Explain

A: In electronic spectroscopy, generally the electron excites  from an initial low energy state to a…

Q: 4. An ethanol solution of p-aminosalicylic acid has a molar absorptivity of 1.6 x 10ʻL mol"em at a…

A: Absorbance can be calculated by the following equation: A=εbc where A: Absorbance of the solution ε:…

Q: extinction coefficient = ε, path length = b, concentration C A sample containing an unknown…

A:

Q: Suppose that a solution has an absorbance of 0.226 at a wavelength of 450 nm.  If the concentration…

A: Given the absorbance of the solution, A = 0.226 Concentration of the solution, C = 24 μM  path…

Q: absorbance of a sample is 0.521, what is its concentration?

A:

Q: ) The refractive index (μ) of water is found to have the values 12.9,12.5,12.3 and 11.8. Calculate…

A: The question is based on the concept of analytical treatment. we have been given refractive indices…

Q: You graph the calibration curve of a solution of an unknown metal (we will call it M+) at 400 nm.…

A: To calculate value of concentration, we can use the graph. For this use the given value of…

Q: In the gold nanoparticle experiment, why did the two gold nanoparticle solutions have different…

A: Option (d) The concentration of tea affected the size of the nanoparticles, which affected the…

Q: What is a calibration curve? O A curve generated by measuring unknown standards and graphing their…

A: Answer - The correct option is (D) A curve generated by measuring known standard and graphing their…

Q: What does the absorbance of a protein at 280 nm reflect and how does that information help you…

A: In UV-Visible spectroscopy light is passed through the sample and if the compound to be analyzed…

Q: Based on the given data, construct and evaluate the calibration curve by plotting absorbance versus…

A: #Calculation for the mean concentration of Copper: Mean absorbance of the diluted (1:10) unknown tap…

Q: You are measuring the concentration of an unknown protein sample, and the absorbance at 595 nm (or…

A: To answer: what would be the best strategy to measure the concentration of your unknown (instead of…

Q: 1. To determine the concentrations (mol/L) of Co(NO3)2 (A) and Cr(NO3)3 (B) in an unknown sample,…

A: Solution - According to the question - Given data - Absorbance of 0.15 M Co(NO3)2 at 510nm =  =…

Q: QUESTION 4 Answer the following questions based on the given table. Compound Chromophores Wavelength…

A: A question based on electronic spectra, which is to be accomplished.

Q: A solution of a specific vitamin has a Âmax of 245 nm and a concentration of 4.15 × 10-/M. The…

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Q: Calculate the specific absorbance ratio (SAR), where A620 = 0.104 and A280 = 1.270. Enter your…

A: The specific absorbance ratio (SAR) is a useful parameter in biochemistry and molecular biology, as…

Q: 6. The equation of Beer Lambert's Law is A = Ebc. If the 'b' is distance, 'E' is absorptivity, and…

A: In Beers - Lambert's equation what does 'c' represent ?

Q: Plot (using appropriate scaling, with titles, axis labels, and units where applicable) the…

A: Beer's law state that absorbance of a solution is directly proportional to concentration of…

Q: What is the molar concentration for a solution with a measured absorbance of 0.212 based on this…

A: According to Beer-Lambert’s law, the absorbance of solution can be expressed as:

Q: A solution has an absorbance reading of 0.238 AU. Using the calibration curve below, determine the…

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Q: A standard curve was performed using an Absorbance spectrophotometer, and the equation of that line…

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Q: What is the correct order of the components in a UV-Visible spectrophotometer?

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Q: How do you calculate the amount of µmoles, if the absorbance is 0.019, the extinction coefficient is…

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Q: For the determination of Fe3 +, 25 mL of sample taken from sea water is diluted to 100 mL and a…

A: A question based on Beer-Lambert law, which is to be accomplished.

Q: wavelength (microns) 2.5 100 89 10 11 13 15 4. 80 60 40 20 4000 3500 3000 2500 2000 1500 1000 625

A: The broad band corresponding to alcohol functional group should appear in the region of (3000-3500)…

Q: The ultraviolet spectrum of aspirin shows a secondary absorption band at 271 nm. If a solution of…

A: The absorbing species obey Lambert beer law

Q: Why should absorbance be less than 2?  Please explain in terms of light transmittance

A: Absorbance A also called the optical density , is the quantity of light absorbed by a solution.

Q: 73 0.3892 4 0.574 0.4568 5 0.673

A: Given:

Q: Determine the concentration of a solution with an extinction coefficient of 82 Mcm and an absorbance…

A: Use Beer-Lambert's Law.A = ebcwhere A = absorbancee = extinction coefficientb = size of cuvettec =…

Q: Time (min)  A  0  0.617  10  0.536  20  0.456  30  0.375  40  0.294  50  0.214  60…

A: Using Beer-Lambert LAw, A= εb * c where A= absorbance, c=concentration (mol/L), ε = molar extinction…

Q: To determine the amount of copper and the identity of the copper compound in the unknown sample, a…

A: A question based on general chemistry that is to be accomplished.

Q: The “SPF number” of a sunscreen increases as the sunscreen absorbs more light and, therefore,…

A: For SPF 2,  Transmittance(T) = 12=0.5 Absorbance(A) = log1T=log 2=0.301 Fraction of UV-B radiation…

Q: A student uses visible spectrophotometry to determine the concentration of CoCl2(aq)CoCl2(aq) in a…

A: This question asks one to find the concentration of a solution where CoCl2(aq) as the solute and…

Q: In spectophotometry, what should be done if there is a greater absorbance reading than the most…

A: The concentration is directly proportional to the absorbance value. That means, the higher the…

Q: if its absorbance is 0.900, find the molar absorptivity of a solution containing 3.0 x 10-3 g/L…

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Q: You want to figure out the caffeine concentration in a coffee you bought. You make four caffeine…

A: The objective of the question is to determine the concentration of caffeine in a coffee sample using…

Q: Draw a plot having X-axis as concentration (mol/L) and Y-axis as absorbance at λ max (525 nm).…

A: 1.  Concentration vs absorbance plot

Q: solution with %T = 75.9% has an absorbance equal to: A = log (1 T) T= (% T 100)

A: Relationship between absorbance and transmittance, A = - log T And Relationship between absorbance…

Q: How does pipetting laboratory techniques affect absorbance values when using UV-Vis…

A:

Q: QUESTION 6 Most analytical machines used in science can either measure absorbance of light, like the…

A: From the given data, the graph is formed as follows: The equation of the graph is y = 1.662 x +…

Q: What is the purpose of using blank before measuring the absorbance of a substance?

A: The question is based on the concept of quantitative analysis. it involves the importance of blank…

Quantum Chemistry

Quantum chemistry deals with the properties of chemical systems and their reactions. The branch of chemistry which is more concerned with quantum chemistry is physical chemistry. It is a more sophisticated field of theoretical physics which is concerned towards the application of physical concepts towards various chemical aspects such as, formation of atoms, chemical structure descriptions, chemical bond strength, hybridization, conjugation etc. Quantum chemistry mainly studies the various states i.e., ground state, excited state, transition states of the individual atoms or molecules taking place during any chemical reaction. The laws of motion explained by various scientists, which are termed as “classical mechanics” were not acceptable in terms of electrons, protons, atoms, light etc. Thus, a new theory named “quantum mechanics” emerged to understand the basic framework of various atomic and subatomic particles.