Please indicate whether the following statement is true or false In eukaryotes, promoter escape involves dephosphorylation of RNA polymerase II enzyme
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- Please indicate whether the following statement is true or false
In eukaryotes, promoter escape involves dephosphorylation of RNA polymerase II enzyme
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- Trp operon of E. coli is an inducible sytem since it turns on in the presence of tryptophan. In most bacteria, protein synthesis is initiated with a modified methionine residue (N-formylmethionine), whereas unmodified methionines initiate protein synthesis in eukaryotes. Both DNA replication and transcription follow a 5’ to 3’ direction of polarity. Write T if the statement is true and write F if the statement is falseWhen referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, ________. tryptophan inactivates the repressor protein translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels the ribosomes stall during translation of the attenuator region transcription termination at the attenuator region is likelyThe diagram below represents the tryptophan operon with the trp leader mRNA transcript enlarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the MRNA leader region. Suppose a mutant bacteria has region 4 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Lead&r region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence MRNA UGGUGG 1 (tryptophan codons) AUG UUUUUU No There is insutficient information to answer the question. O Yes
- The following diagram represents one of the Christmas-tree-like structures shown in Figure On the diagram, identify parts a through i. Q. Approximate location of the promoterThe diagram below represents the tryptophan operon with the trp leader mRNA transcript enlarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the mRNA leader region. Suppose a mutant bacteria has region 3 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Leader region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence MRNA AUG UGGUGG UUUUUU 1 2 3 (tryptophan codons) There is insufficient information to answer the question. Yes No O ONegative supercoiling of DNA favors the transcription of genes because it facilitates unwinding. However, not all promoter sites are stimulated by negative supercoiling. The promoter site for topoisomerase II itself is a noteworthy exception. Negative supercoiling decreases the rate of transcription of this gene. Propose a possible mechanism for this effect and suggest a reason why it may occur.
- e B 1_30*_SP23 - General Biology I (for majors)/11364 of € 2 A us page X F1 What conditions would we find on the gene of a prokaryote if there is low amounts of tryptophan within the cytoplasm? Select one: a. lactose would attach to the repressor removing it from the promoter promoting the transcription of lactase b. no repressor is on the promoter creating constant transcription of lactase c. tryptophan would attach to the repressor removing it from the promoter promoting the transcription of lactase O d. repressors would bind to the promoter stopping the transcription of lactase e. lactose would attach to the repressor removing it from the promoter promoting the transcription of tryptophan producing enzymes no repressor is on the promoter creating constant transcription of tryptophan producing enzymes tryptophan would attach to the repressor binding it to the promoter and stopping the transcription of tryptophan producing enzymes O f. O g. 2 Oh. tryptophan would attach to the…Which of the following statements is/are TRUE for the promoters? The Pribnow box and TATA box are found in the prokaryotes and eukaryotes, respectively. For both prokaryotes and eukaryotes, the first ribonucleotide attaches to the TSS region. The -35 element in prokaryotes is called the Up element in eukaryotes. In prokaryotes, the Pribnow box is located 25 bases before TSS.Which one of the following amino acids is phosphorylated during the process of promoter clearance by eukaryotic RNA polymerase II? Othreonine tyrosine serine Oproline
- Imagine you are going to label a gene associated with apoptosis in Symbiodiniaceae with a Yellow Fluorescent Protein (YFP). To generate the YFP, you know the pre-MRNA looks as follows: Unspliced YFP premature mRNA Сap 5' UTR Exon 1 Intron Exon 2 Intron Exon 3 3' UTR Poly-A tail If Exon 2 is also required for mRNA stability, what can be predicted from the possible spliced alternative isoforms formed? One of the isoforms will not have a poly-A tail O The alternative splicing of YFP pre-MRNA prevents 5'-capping The MRNA isoform without Exon 2 will be degraded faster than the other isoform Exon 2 will be added to isoform B later to correct the mistake in splicing The protein translated from one of the mRNA isoforms will possess an additional functional domainWhich one of the following amino acids is phosphorylated during the process of promoter clearance by eukaryotic RNA polymerase II? tyrosine threonine serine prolineThe trp operon is: **(for additional information review the CogBooks Module, answer explained on CogBooks): In the absence of tryptophan, the trp repressor dissociates from the operator, and RNA synthesis proceeds. promoter operator trpE| trpD trpC trpB trpA RNA polymerase repressor When tryptophan is present, the trp repressor binds the operator, and RNA synthesis is blocked. promoter operator trpE trpD trpC trpB trpA RNA polymerase; repressor tryptophan Both negative inducible and positive repressible Positive inducible Negative Repressible O Negative inducible