EXPERIMENT 4: INDUCTION OF CALLUS SOMATIC EMBRYOGENESIS OF HAPLOID PLANTSObjective:To prepare anther as a source of explantTo induce callus somatic embryogenesis of haploid plantsProcedure:Cut off the buds and sort them into 3 developmental stages based on length of buds Surface sterilize the buds in 70% ethanol for 2-3 minutes, making sure the entire bud is immersed in the alcohol. Drain off excess alcohol from the buds and aseptically excise the anthers from each bud. Remove the filaments and culture the anther on one of the agar media provided. Label the stage of development of the bud on each petri dish as the anthers are cultured. Seal the Petri dishes with parafilm. Incubate in the dark at 26 to 28ºC for 4 to 8 weeks or until small plants can be seen growing out of the anthers. Then transfer to diffuse light. Record the results of the experiment in table form and submit the report. Observation: The culture was contaminated after 8 weeks of incubation QUESTION: Please give me a brief discussion on the observation (i.what can you observe, ii. what are the source(s) of contamination in this experiment? iii. how to prevent this contamination from happening?)

Plant tissue culture is a modern technique employed to get a desired variety of plants from any…

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Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter33: The Plant Body

Section: Chapter Questions

Problem 14TYK

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EXPERIMENT 4: INDUCTION OF CALLUS SOMATIC EMBRYOGENESIS OF HAPLOID PLANTS

Objective:

To prepare anther as a source of explant
To induce callus somatic embryogenesis of haploid plants

Procedure:

Cut off the buds and sort them into 3 developmental stages based on length of buds
Surface sterilize the buds in 70% ethanol for 2-3 minutes, making sure the entire bud is immersed in the alcohol.
Drain off excess alcohol from the buds and aseptically excise the anthers from each bud.
Remove the filaments and culture the anther on one of the agar media provided. Label the stage of development of the bud on each petri dish as the anthers are cultured. Seal the Petri dishes with parafilm.
Incubate in the dark at 26 to 28ºC for 4 to 8 weeks or until small plants can be seen growing out of the anthers. Then transfer to diffuse light.
Record the results of the experiment in table form and submit the report.

Observation:

The culture was contaminated after 8 weeks of incubation

QUESTION: Please give me a brief discussion on the observation (i.what can you observe, ii. what are the source(s) of contamination in this experiment? iii. how to prevent this contamination from happening?)

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