One method for separating polypeptides makes use of their different solubilities. The solubility of large polypeptides in water depends on the relative polarity of their R groups, particularly on the number of ionized groups: the more ionized groups there are, the more soluble the polypeptide. Which of each pair of polypeptides that follow is more soluble at the indicated pH?(a) (Gly)20 or (Glu)20 at pH 7.0(b) (Lys–Ala)3 or (Phe–Met)3 at pH 7.0(c) (Ala–Ser–Gly)5 or (Asn–Ser–His)5 at pH 6.0(d) (Ala–Asp–Gly)5 or (Asn–Ser–His)5 at pH 3.0
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
One method for separating polypeptides makes use of their different solubilities. The solubility of large polypeptides in water depends on the relative polarity of their R groups, particularly on the number of ionized groups: the more ionized groups there are, the more soluble the polypeptide. Which of each pair of polypeptides that follow is more soluble at the indicated pH?
(a) (Gly)20 or (Glu)20 at pH 7.0
(b) (Lys–Ala)3 or (Phe–Met)3 at pH 7.0
(c) (Ala–Ser–Gly)5 or (Asn–Ser–His)5 at pH 6.0
(d) (Ala–Asp–Gly)5 or (Asn–Ser–His)5 at pH 3.0
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