Answered: ОН CH3—С—СОо + NADH + H+ CH3-C-CO0¯ +…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

The muscle enzyme lactate dehydrogenase catalyzes the reaction NADH and NAD⁺ are the reduced and oxidized forms, respectively, of the coenzyme NAD. Solutions of NADH, but not NAD⁺, absorb light at 340 nm. This property is used to determine the concentration of NADH in solution by measuring spectrophotometrically the amount of light absorbed at 340 nm by the solution. Explain how these properties of NADH can be used to design a quantitative assay for lactate dehydrogenase.

ОН
CH3—С—СОо + NADH + H+
CH3-C-CO0¯ + NAD+
Pyruvate
H
Lactate

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