Of the two principal polymers used in column chromatography and electrophoresis, which one would be most immune to contamination by bacteria and other organisms?

Q: Why is the order of separation based on size opposite for gel filtration and gel electrophoresis,…

A: Gel filtration and gel electrophoresis are biochemical techniques used for the separation of…

Q: Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.

A: Slants, Butt-slants, and butts are different types of media prepared for bacterial growth. Slants…

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…

A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…

Q: In which type of chromatography, solvents of increasing polarity are passed through a column of…

A: Chromatography is a separation technique that is used to separate components of a mixture based on…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: eservatives cannot be used for direct + mount ?

A: The disease is a state of the body in which the physiological mechanisms do not function in a normal…

Q: What factor allows molecules to be separated by chromatography?

A: To find The factor that allows molecules to be separated by chromatography

Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?

A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…

Q: Which of the following cannot be grown in artificial media and must be grown in live animals or…

A: Artificial media is a medium that is defined. This medium is chemically defined. It is a synthetic…

Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…

A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…

Q: Why does water separate out from a gel after freezing?

A: On freezing of gel some damage occur to gel. damage occur to polymer and colloids that binds the gel…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: Under what conditions are media typically sterilized in an autoclave?

A: An autoclave is the pressure chamber used to carry out the scientific and industrial processes…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: List three coating techniques used to make sol-gel coatings, and briefly describe each technique.

A: Sol-gel  Is a method of creating solid materials from tiny molecules.  The process involves the…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: How can polarography detect the analyte?

A: Jaroslav Heyrovsky discovered polarographic method   Polarography is a simple method in analytical…

Q: What are two types of compounds that make up the resin for column chromatography?

A: Column chromatography is a technique used for the separation or purification of biomolecules such as…

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: What types of macromolecules are usually separated on agarose electrophoresis gels?

A: Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular…

Q: Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation?

A: Introduction Microbial culture is a microbiological technique that involves actively multiplying…

Q: What is the difference between an antiseptic and a disinfectant? Can the same chemical be used for…

A: Antiseptic Disinfectant 1.       These can slow down the growth as well as can kill the…

Q: Of what practical importance are air borne microorganisms to the laboratory workers?  What…

A: Questions related to the microbiology labs.  Note: each questions are answered under particular…

Q: If a technician examined a Kirby-Bauer assay and found individualcolonies of the plated microbe…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…

A: The preparing a slide that has to be observed under a microscope there are multiple steps that are…

Q: Chromatography technique works on the principle of

A: Chromatography is a technique used for separation of mixture by using a moving solvent.

Q: why would you perform an indole test?

A: The inside test is a qualitative approach of the biochemical analysis of bacteria that determine…

Q: Can paper chromatography be used to separate and identify very volatile substances? Explain your…

A: Paper chromatography is a separation technique that is mainly involved in the principle for the…

Q: For microprojectile bombardment, the particles used are typically? a. Nickel b. Tungsten O c.…

A: Ttransformation is a biotechnological process where a foreign DNA molecule is inserted into the host…

Q: What is meant by the term plating efficiency?

A: The cells are cultured over the plate at the particular growth conditions within a particular time…

Q: How might a synthetic detergent be superior to a normal soap?

A: INTRODUCTION - Soaps are  sodium or potassium fatty acid salts made from hydrolysis of fats in…

Q: What types of compounds make up the gels used in electrophoresis?

A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…

Q: in paper chromatography what is the basis of pigments separate?

A: Paper chromatography is a technique in which different types of pigments can be separated from each…

Q: What are the advantages of solid media  ?

A: Microbial culture is a method of multiplying microbes by allowing them to reproduce in a…

Q: How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml…

A: Serial dilution of the original sample containing microorganisms to achieve countable colonies on…

Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?…

A: In dark-field microscopy, microbes appear light in a dark background., viruses, macromolecules,…

Q: Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?

A: INFILTRATION is the process in which the clearing agent is completely removed from the tissue and…

Q: bacteria require staining before microscopic examination?

A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…

Q: What products are sterilized using high energy ionizing radiation?

A: Sterilization is the process of killing microorganisms.

Q: Why is cobalt chloride paper used in the experiments?

A: The indicators are used in the experiment to detect the distinct observable change. Many types of…

Q: Which of the following scientists use this X-ray crystallography?

A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…

Q: What is the difference between a tincture and an aqueous solution?

A: Given: Explain the difference between a tincture and an aqueous solution

Q: Why can't we determine Vo, Ve and Vt for affinity chromatography?

A: Chromatographic techniques separate macromolecules based on size, ion exchange, or their affinity…

Q: What are the advantages and disadvantages of liquid media?

A: Microbiological culture is a method of multiplying microorganisms in lab conditions on growth media.