Nucleic Acids.p... Direction 1: Problem Solving. Provide systematic and legible solutions to the following problems: 1. Draw the following DNA segment 5' C-A-T 3' NH, 3 H CH3 N° `N' 3 H H H Pyrimidine Purine Thymine (T) Cytosine (C) Uracil (U)

1. complete the table below: Volume Procedure (mL) Crude 120 extract GFC 20 IEX 10 Protein Total Unit Total Specific content protein Activity Activity Activity (mg/mL) (mg) (U/mL) (U) (U/mg) 20 (a) (d) 0.356 (h) 11 (b) (e) 0.207 (i) 9 (c) (f) (g) (j) % Purification Recovery fold (k) (1) (n) (m) (0)

The vast majority of structures deposited in the Protein Databank (>95%) have been determined using NMR   Hard keratins (such as in the skin) are less resistant to stretch because the larger number of disulfide bonds resist deformative forces.   Structures produced by X-ray diffraction are as good as the solutions from which they are derived     they are false and I know that but explain why they are false

6. b. Draw a box around the disulfide bridge in oxytocin, if present, or write "none". 7. Mark each peptide bond in oxytocin by making the corresponding line in the structure thicker or marking it with a different color. The first one is shown for you as an example (in dark orange). 8. Number the central carbon of each amino acid in oxytocin by pointing a small arrow to it or by circling the corresponding vertex in the image. Numbers 1 and 2 indicate the central carbons of the first and second amino acids of oxytocin, and are shown for you as an example. 9. Fill out the following table, listing amino acids that make up oxytocin in order, from the N terminus to the C terminus, characterizing each amino acid by the properties of its R group (side chains), and briefly indicating the reasoning for the characterization. You may consult amino acid groupings by category in the slides (or the textbook, p.49), but you must explain the reasoning for each in your own words. CO 1 AA# Abbre- Full…

Penicillin G is a natural antibiotic that is useful for treating infections caused by Gram positive bacteria. What is the functional benefit of the semi-synthetic antimicrobials carbenicillin and ampicillin, generated by chemical modification of the R group so their R groups each are a bit different than the R group seen with penicillin G?

only need z

Which one of the following statements about nucleic acids is correct? Multiple Choice NTPs can be incorporated into either DNA or RNA molecules, but dNTPs can only be incorporated into DNA molecules A nitrogenous base, 5-carbon sugar, and two phosphate groups would most accurately be referred to as a nucleotide diphosphate. During nucleic acid synthesis, the phosphate group at the 3' carbon of the incoming monomer is covalently linked to the hydroxyl group at the 5' carbon of the existing macromolecule. RNA and DNA have all the same pyrimidine bases in them, but differ in the selection of purine bases. A nucleic acid found in a cell with the sequence GCCTTATCGCG would most likely be found paired with another molecule in double-stranded form.

5. A mixture containing phenylalanine, tryptophan, and leucine was subjected to electrophoresis. Determine which of the amino acids moved the farthest distance assuming that the experiment was performed at the pH indicated: (a) pH 6.0 (b) pH 5.0

I'm very confused on #13. Can anyone explain if the claim is valid or not? I don't understand how to calculate #15. Can someone show the formula for it? #16 I think is C and #17 I think is B. Is this correct?

1. You need 0.5 liter of a 0.1 M buffer for a biochemical reaction that you plan to perform at pH 2.0. On the shelf in your laboratory you have available phosphoric acid, and the amino acids aspartate, glycine, glutamate, tyrosine, and histidine (all you have available are the fully deprotonated forms of the molecules); these are the only buffer materials you have available. (The pKa values for these compounds are given below.) For phosphoric acid, pKal1 = 2.12, pKa2 = 7.21, and pKa3 = 12.32 For aspartate, pKaCOOH = 1.88, pKaNH3+ = 9.60, pKa sidechain = 3.65 For glycine, pKaCOOH = 2.34, pKaNH3+ = 9.60 For glutamate, pKaCOOH = 2.19, pKaNH3+ = 9.67, pKa sidechain = 4.25 For histidine, pKɑCOOH = 1.82, pKɑNH3+ = 9.17, pKa sidechain = 6.00 For tyrosine, pKaCOOH = 2.20, pKaNH3+ = 9.11, pKa sidechain = 10.07 a. Of these possible buffers, which is likely to yield the greatest resistance to pH change from the starting pH of 2.0, assuming that you were going to run a biochemical reaction that…