Name the three temperature-dependent steps of the PCR method and explain the key event(s) associated with each of those.

what are ways you can run a PCR cycle in a single test tube? how would you adjust amplificons, enzymes, ddNTPs, Mg concentration so that it would be successful?

A student is trying to add 9.0 pmol of primer mix to a 20.0 µL PCR.  The primer mix is at a concentration of 50.0 µM, and the student determines that a serial dilution is required because directly adding the primer mix would require a volume less than 1.00 µL.  The student takes 1.40 µL of the primer mix stock and mixes it with 25.0 µL of ultrapure H2O.  Calculate the volume (in µL) of the intermediate solution required to add the correct amount of primer mix to the PCR.

1) Describe the ingredients of a PCR reaction.  List different temperatures used in each cycle and tell why each is used.