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You can perform a specific staining method to confirm the purity on the culture. Describe
this staining method.
Step by step
Solved in 2 steps
- The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainWhy is there a need to use a staining agent in observing the specimen?Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
- If a spectrophotometer is available, dispense the culture into labeled corvette and determine the optical density of each cultureDescribe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at every step of the Gram staining procedureList the primary stain, secondary stain, mordant, and decolorizer for the gram stain, and write them in the order that you would use them. Make sure to label which is which.
- Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______. Positive cells that retain the primary stain are ______, while negative cells are counterstained with _____ and will have a ______ color. *Please use answers from the options provided in the image below.In outline form discuss the steps of Ziehl-Neelsen staining Complete the table for National Reporting Scale (Description) National Reporting Scale Result Description 0 +n 1+ 2+ 3+
- Choose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with waterc: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what you see. Which of the following tests would you use next? 中 МАС O HE • EMB O MSA