Most of the regulatory systems described in this chapter employregulatory proteins. However, regulatory RNA is also important.Describe how one could achieve negative control of the lacoperon using either of two different types of regulatory RNA.

Explain how the trp operon is regulated by trp repressor and by attenuation.

Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to the Group of answer choices Operon Operator Repressor Promoter RNA polymerase

Wilms tumor 1, or nephroblastoma, is caused by mutations in the WT1 gene, which encodes a transcription factor. You have identified a novel variant in WT1: Arg422Pro.   You have control cells and cells that have been engineered to carry the homozygous WT1 p.Arg422Pro mutation. You want to assess effects of this mutation on a variety of endpoints. For each endpoint listed below, choose the one technique is best suited to answer the question.   Choose from: array CGH, qRT-PCR, qPCR, RNA-seq, FISH, in situ hybridization, western blot, immunostaining, WT1 ChIP-seq, WT1 ChIP-PCR, ATAC-seq, 3C     Endpoint Technique? WT1 protein amount (quantitative) Western blot WT1 protein binding to all enhancers, genome-wide Chip-seq WT1 mRNA amount (quantitative)   WT1 protein subcellular localization   Quantitative assessment of all mRNAs in these cells (genome-wide) RNAseq Chromatin interactions between a specific WT1 chromatin binding site (identified above)…