Make a flowchart for the procedure of SPREAD PLATE METHOD

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.8: Laboratory Math And Reagent Preparation
Problem 2.1CS
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Make a flowchart for the procedure of SPREAD PLATE METHOD

C. SPREAD PLATE METHOD:
a. Pour melted NA or CWA into each of six sterile petri dishes and allow to solidify.
b. Transfer to duplicate plates 0.1 ml from each tube used in pour plate technique.
c. Sterilize an L-shaped glass rod by dipping in alcohol and flaming until alcohol has
burned off.
d. Spread the inoculum evenly on the surface of the agar with an L-shaped rod.
Transcribed Image Text:C. SPREAD PLATE METHOD: a. Pour melted NA or CWA into each of six sterile petri dishes and allow to solidify. b. Transfer to duplicate plates 0.1 ml from each tube used in pour plate technique. c. Sterilize an L-shaped glass rod by dipping in alcohol and flaming until alcohol has burned off. d. Spread the inoculum evenly on the surface of the agar with an L-shaped rod.
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