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- Procedure Explant Preparation and Inoculation 1) The leaf explants were cut into squares (approximate size = 1cm x 1cm ). 2) The explants were soaked in 95% EtOH for 3 minutes with constant shaking. 3) EtOH was then decanted and 10% of Chlorox bleach was added together with a few drops of Tween 20, with constant shaking. 4) The explants were rinsed with autoclaved distilled water for at least 5 times. 5) The explants were then left to dry in the laminar air flow cabinet. 6) The explants were cultured in the appropriate media (2 explants in basal MS without BAP and the other two in MS with BAP). 7) Three explants were placed on its upper surface down and the other three with lower surface down in both basal MS media with and without BAP. 8) All the culture jars (name, date and treatments employed etc.)were labelled and incubated them (in dark) in the growth chamber or culture room. 9) Explants were placed on the surface of the medium and the Petri dish were sealed with parafilm. 10)…Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?Questions 1. Explain why it was important to transfer the smallest colonies of yeast to the selective media plates.
- Bacterial generation times for four different bacterial species were calculated in the media listed in Table 6.3. All media were prepared with pure distilled water and incubated aerobically in the light. Compare and contrast the growth requirements of the four bacteria listed above. Which of the media, if any, are chemically defined?| Generation Time Escherichia coli Pseudomonas Lactobacillus Nitrobacter Medium aeruginos a NaCl, NO3", MgSO4 80 Glucose 100 Glucose, NaCl, PO43- Glucose, NaCl, PO43-, MgSO4 Glucose, NaCl, PO4-, MgSO4, 56 200 43 100 28 40 8 amino acids Glucose, NaCl, PO43-, MgSO4, 25 25 80 19 amino addsDefine the following termonologies in brief 1.Cellular Permeability 2.Fluorescence in situ Hybridization(FISH) 3. Genotoxicity 4.Medical Device 5. SterilizationTopic: Yeast cells (dalmau plates) What are the principles of PDA, AcA, and MA plates in media preparation? please explain all
- TOPIC : MICROBIAL GROWTH Classify the bacteria that is most likely involved in each situation provided according to their classification (as to microbial growth requirements). CHOICES: A. Obligate aerobe ,B. Obligate anaerobe, C. Microaerophile, D. Aerotolerant anaerobe, E. Mesophile, F. Thermophiles 1.The bacteria under study thrives at the alveolar ducts in the lungs causing respiratory disease. 2.In the experiment, the agar plate containing an axenic culture was originally incubated inside a candle jar for one day and then transferred to an oxygen-rich incubator for another day. Findings: the microbial growth persisted and stayed the same regardless of the specific type of incubation employed. 3.A microorganism that can survive pickling and refrigeration was discovered when a tightly-sealed jar of pickles grew several colonies despite following the correct recipe, tight closure seal, and proper refrigeration. 4.The spoiled bottle of wine that was stored at standard room temperature…6:51 Done Edit This microbe does not have a fermentation pathway sufficient for growth when oxygen is not present O Obligate anaerobe O Aerotolerant py O Obligate aerobe ear O Microaerophile MoreTitle: Spoilage of coconut water Aim: To assess the diversity of psychrotrophic bacteria causing spoilage in coconut water. How might incubation of plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?
- EXPERIMENT:EFFECT OF CARBON SOURCES ON THE MICROBIAL GROWTH. 1.OBJECTIVE To study the effect of media components on microbial growth 2.PROCEDURES Preparation of fungal suspension inoculums Prepare the laminar cabinet for the inoculum preparation by applying normal aseptic techniques To a full-grown fungal Petri dish, add 25ml sterilized distilled water and rub the agar gently with glass rod Pour the suspension into an empty flask and continue previous steps to achieve your desired volume of inoculums suspension. Cotton-plugged and aluminium-wrapped the mouth of inoculum flask. For certain fungal species like Niger, the suspension from the agar should be filtered upon pouring into the inoculum’s flask. Preparation of salt media of different carbon sources. Prepare the Modified Czapek Dox media by weighing the following salts according to your desired working volume. KH2PO4 (1 g/L) NaNO3 (3 g/L) MgSO4.7H2O (0.5 g/L) Carbon source (30 g/L) KCl (0.5 g/L) 2.…Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain When is sterilization by filtration preferred over sterilization by heat? Why?