Joseph, being a know-it-all, told Mary that her method would not include all microbes. To demonstrate this, he took another 10g sample of Mary's garden and added starch. After the 1-week incubation time, he performed the same serial dilution scheme as Mary but used pour plating instead of spread plating. Shown below are the counts that he obtained after the incubation period. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures. Tube 1 135, 129 Tube 2 9,7

Joseph, being a know-it-all, told Mary that her method would not include all microbes. To demonstrate this, he took another 10g sample of Mary's garden and added starch. After the 1-week incubation time, he performed the same serial dilution scheme as Mary but used pour plating instead of spread plating. Shown below are the counts that he obtained after the incubation period. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures. Tube 1 135, 129 Tube 2 9,7

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter3: Basic Hemostasis

Section3.3: Principles Of Coagulation Testing

Problem 4RQ

See similar textbooks

Similar questions

Joseph, being a know-it-all, told Mary that her method would not include all microbes. To demonstrate this, he took another 10g sample of Mary's garden and added starch. After the 1- week incubation time, he performed the same serial dilution scheme as Mary but used pour plating instead of spread plating. Shown below are the counts that he obtained after the incubation period. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures. Tube 1 135, 129 Tube 2 9.7
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?
Please note that these counts have all been done with Trypan Blue. Therefore, all cultures have been diluted two-fold (equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.1These numbers represent the total number of cells counted in 5 large squares from three separate counts.
The student advises using the same selection procedures and inoculating a 5 mL overnight culture with the transformed cells instead of searching for transformed coli on LB agar plates in order to save time. Why isn't this a wise decision?
Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample, added urea, and then let it incubate for one week. She then performed serial dilution a shown below: 10 g soil sample To: 1ml 90 ml Dilution Bottle 1 ml 9 ml Tube 1 0.1 ml 0.45 ml 111 9.9 ml Tube 3 9 ml Tube 2 9.55 ml Tube 4 Calculate the dilution of the bottle and each tube. Be sure to show complete solutions and box your final answer.
You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar.
A culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?
There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectively
You were tasked to prepare four petri plates. Three of the petri dishes were inoculated using different samples. The plates were prepared from the following: Plate A: oral swab after brushing Plate B: cat’s paw Plate C: exposed in air for one hour Plate D: uninoculated The plates were incubated at 37˚C for 24 hours. The following results were obtained: 1. Compare the growth of the cultures found in Plates A, B, C and D. Which among the four plates has shown the most growth? Why do you think so? 2. Describe the appearance and abundance of the colonies found in your four plates. What possible type of microorganisms are found in your plates? 3. Although sparse, there is still visible growth of microorganisms in Plate D (uninoculated plate). Where do you think the microorganism came from? REFERENCES:
Which of the following gives a positive result for acrolein test?*
A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.