DNA primase synthesizes a short RNA primer that later appears at the 5'-end of each Okazaki fragment.

Provide the sequences of the template and coding strands of a DNA double helix that was used toproduce this RNA: 5’-GUUACCGACUAU. Be sure to label the 5’ and 3’ ends.

The overall structures of RNA polymerase and DNA polymerase are very different, yet their active sites show considerable similarities. What do the similarities suggest about the evolutionary relationship between these two important enzymes?

Introns in protein-coding genes of some eukaryotes are rarely shorter than 65 nucleotides long. What might be a rationale for this limitation?

If the RNA polymerase II subunits are in the same family, why would they have such little similarity to each other? Which RPB proteins were most closely related to each other? Why?

Hundreds of DNA double strand breaks are created by SPO11, a topoisomerase type II like protcin. (i) Draw and explain how SPO11 can generate double strand breaks.

In reversible terminator sequencing, how would the sequencing process be affected if the 3′-end-blocking group of each nucleotide were replaced with the 3′-H present in the dideoxynucleotides used in Sanger sequencing?

Why would you NOT expect a restriction endonuclease to exist that would recognize the site AAGGAA?

You isolate a mouse Tau-gene-containing DNA fragment from the chicken and hybridize it to the freshly-made and isolated hnRNA (primary transcript) from the nucleus of the mouse cells transcribed from the Tau gene (immediately after it was produced), allowing no time for processing of the hnRNA. Describe what you see when you look at the DNA/RNA hybrid molecule under the electron microscope.

What is the difference between primase and DNA polymerase alpha?