In E. coli, when glucose is present, galactose is used to make a component of the bacterial cell wall. When glucose is absent, galactose is MOSTLY used to make energy, but a little is used to make the cell wall. The enzymes for the conversion of galactose into a cell wall component are transcribed as an operon. The operon is transcribed at a high level when glucose is present and at a low level when glucose is absent. You isolate mutants that do not respond appropriately to the presence or absence of glucose, as summarized below. Galactose incorporation into cell wall strain + Glucose Glucose Wild type High High High Low Mutant 1 High High Mutant 2 Mutant 3 Low Low Mutant 4 None None Mutant 5 High None Mutant 6 None None Which of the above mutants could have a mutation in the promoter for the operon with enzymes for galactose incorporation into cell wallI?

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In E. coli, when glucose is present, galactose is used to make a component of the bacterial
cell wall.
When glucose is absent, galactose is MOSTLY used to make energy, but a little is used to
make the cell wall.
The enzymes for the conversion of galactose into a cell wall component are transcribed as
an operon.
The operon is transcribed at a high level when glucose is present and at a low level when
glucose is absent.
You isolate mutants that do not respond appropriately to the presence or absence of
glucose, as summarized below.
Galactose incorporation into cell wall
strain
+ Glucose
- Glucose
Wild type
High
High
High
Low
Mutant 1
High
High
Mutant 2
Mutant 3
Low
Low
Mutant 4
None
None
Mutant 5
High
None
Mutant 6
None
None
Which of the above mutants could have a mutation in the promoter for the operon with
enzymes for galactose incorporation into cell wall?
Mutant 3
Mutant 2
Mutant 5
Mutant 1
Transcribed Image Text:In E. coli, when glucose is present, galactose is used to make a component of the bacterial cell wall. When glucose is absent, galactose is MOSTLY used to make energy, but a little is used to make the cell wall. The enzymes for the conversion of galactose into a cell wall component are transcribed as an operon. The operon is transcribed at a high level when glucose is present and at a low level when glucose is absent. You isolate mutants that do not respond appropriately to the presence or absence of glucose, as summarized below. Galactose incorporation into cell wall strain + Glucose - Glucose Wild type High High High Low Mutant 1 High High Mutant 2 Mutant 3 Low Low Mutant 4 None None Mutant 5 High None Mutant 6 None None Which of the above mutants could have a mutation in the promoter for the operon with enzymes for galactose incorporation into cell wall? Mutant 3 Mutant 2 Mutant 5 Mutant 1
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