In DNA technology, the term vector can refer to(A) the enzyme that cuts DNA into restrictionfragments.(B) the sticky end of a DNA fragment.(C) a SNP marker.(D) a plasmid used to transfer DNA into a living cell.

Human DNA and a particular plasmid both have sites that are cut by the restriction enzymes HindIII and EcoRI. To make recombinant DNA, the scientist should (a) cut the plasmid with EcoRI and the human DNA with HindIII (b) use EcoRI to cut both the plasmid and the human DNA (c) useHindIII to cut both the plasmid and the human DNA (d) a or b (e) b or c

A DNA polymerase enzyme inherently incorporates an incorrect nucleotide at a low but measurable rate. Such a mutation is termed _______. A)  spontaneous B)  lysogenic C)  transformative D)  transductive E)  induced

GENETICS if/when a "whole-genome shotgun" approach is used for DNA sequencing, which of the following is MOST likely to create problems during the assembly of a complete genomic sequence? a) long sequence reads b) a high degree of coverage/ redundancy in the sequence data c) the presence of repetitive DNA d) not enough contigs e) all of the above

From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistry

You discover an E. coli mutant that has a non-functional DNA polymerase III. Such a bactèria; a) Would not be able to synthesize new strands of DNA b) Would not be able synthesize the leading strand of DNA Oc) Would produce new strands of DNA still containing short RNA primers Od) Would be able to synthesize the lagging strand of DNA only

What occurs during the HIGH temperature step of PCR? A) DNA is cut by restriction endonucleases B) primers get degraded C) primers anneal to single-stranded DNA D) DNA is synthesized E) double stranded DNA separates into single stranded DNA

The activity of restriction enzymes may produce fragments with sticky ends.  Sticky ends are   a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?I) Transform bacteria with recombinant DNA molecule.II) Cut the plasmid DNA using restriction enzymes.III) Extract plasmid DNA from bacterial cells.IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.V) Use ligase to seal plasmid DNA to non-plasmid DNA     A. III, II, IV, V, I       B. I, II, IV, III, V     C. III, IV, V, I, II   D. II, III, V, IV, I

Why must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragment