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In aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to incubation?
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- Why is the looping out method preferable for sputum specimen for acid fast smears?Heat-labile material to be introduced to culture medium was filtered using membrane filters with unknown pore size. Effect: Rationale:The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals", describes which technique?
- Loop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate. Effect: Rationale:What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeduring inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.
- Define the following terms; Sterilization, and Aseptic Technique. What are the differences between the two concepts?procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland standard solution is ideally used for inoculation of plated for Kirby Bauer test. a)True b)False Principle of Kirby Bauer test is the diffusion of antibiotic agents in to Agar based media. a) True b) False