The plot shows a correlation between sequence error rate and cluster density on an Illumina flow cell. From your knowledge of how Illumina sequencing works, why do you think there is this correlation?
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The plot shows a correlation between sequence error rate and cluster density on an Illumina flow cell. From your knowledge of how Illumina sequencing works, why do you think there is this correlation?
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- 4.08 2.54 H H 1.02 1.02 1.67 E E = EXON = INTRON E 10.8 kbp 3.94 3.66 E = EcoRI site wwwwwwwww H = Hindill site HI Н 10.31t E E 1.76 1.10 Note: Fragment sizes are not to scale and all fragment lengths are in kilobase pairs (kbp) The a. Indicate what enzymes you would use for digestion and the gel electrophoresis pattern of the restriction fragments (draw a diagram of the gel with fragment positions, indicate field polarity, and direction of migration). Please do single and a double digest of the DNA. GrammarlyWhat are Okazaki fragments and how they are formed?272-2 - 6. The protein assay kit that we will use to quantitate horseradish peroxidase requires absorbance measurements at 562 nm. What type of radiation is this? CCT CCT CCT GOT CCT CCT C Read through the document describing the BCA Protein Assay.? This document is posted separately on Blackboard. What is the protein used in the assay? Why is the assay protein used instead of horseradish peroxidase? 7.
- Agarose gel clectrophoresis is a technique used to separate DNA fragments on an agarose matrix. The DNA is then viewed by staining or under UV light.1068 (i) What is the direction of DNA migration in electric field on the agarose gel? (ii) Explain the principle for the migration of DNA molecules in an electric field. (iii) DNA loading dye is used to prepare DNA markers and samples for loading on the agarose gel. State the function of bromophenol blue in the loading dye.61 identify the primer 3' 5' a 5' 3' C e f a 5' 3' 3' 5' Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a a b b e f a Save UnansweredExpected 260/230 values for "pure" DNA are commonly in the range of
- 1.8 Calculations for the Purification of a Recombinant Protein The purification of a recombinant protein is carried out starting with 100 liters of a clarified cell lysate (i.e., the cells have been lysed, and the cell debris has been removed to give a clarified solution), which has a total protein concentration of 0.36 mg/ml and a recombinant protein con- centration of 2.2 U/ml, where U denotes units of biological activity of the recombinant protein. It is known that the completely pure recombinant protein has a specific activity of 40.0 U/mg. Purification is continued until a chromatography step that yields 2.0 liters of a fraction containing the protein, with a total protein concentration of 1.11 mg/ml and a recombinant protein concentration of 43.2 U/ml.For the recombinant protein, calculate the starting and ending purity, the starting and ending specific activity, and the percentage yield and fold purification through the chromatography step.Question 5. Read mapping is typically the most computationally-intensive step in RNA-seq data analysis. What features of an RNA sequence read and/or the genome might make read alignment take more time? Would you expect this step to take longer when mapping to the Arabidopsis genome or to the human genome? Why?Fluorescence confocal microscopy (FCM) - STK38 monoclonal antibody (M01), clone 2G8- 1F3. a. b. 200 μm What is the basic principle of image formation using this microscopy technique? What can be observed and concluded from the image of the specimen?
- Question: Which base (A, C, T or G) corresponds to X in the unknown? I did the experiment, got the data below, and calculated the binding constants. But I am TOTALLY lost as to how to figure this out! I don't even know what steps I would take. Base pairs Data – all had Temp = 250C PH = 7 Binding Constant A & X [A] = 0.00373221M [X] = 0.00373221M [AX] = 0.0462678M 3.322 C & X [C] = 0.0469007M [X] = 0.0469007M [CX] = 0.00309935M 1.409 T & X [T] = 0.0452279M [X] = 0.0452279M [TX] = 0.00477212M 2.333 G & X [G] = 0.0469554M [X] = 0.0469554M [GX] = 0.00304456M 2.633A typical bacterial DNA has a molar mass of 4 × 109 g m ol-1 . Approximately how many nucleotides does itcontain?2.21 The two allelic fragments of DNA shown belowa (d) After 30 cycles of O (b) Diagram the gel pattern you would expect to amplified by PCR, and the products digested with d St (a) What will the total sizes of the two PCR are JorX BA both PstI and Hind3. products be? from the doubly digested DNA.