If you need to make a lot up to 1 billion copies of a piece of DNA you would use 1. gel electrophoresis  2. cell culture 3. Southern blotting 4. yeast 2 hybrid system 5. polymerase chain reaction

Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.

Which technique rapidly replicated specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reaction

Bacterial transformation is a major concern in many medical settings. Why might health care providers be concerned? Pathogenic bacteria could introduce disease-causing genes in non-pathogenic bacteria Antibiotic resistance genes could be introduced to new bacteria to create “superbugs. ” Bacteriophages could spread DNA encoding toxins to new bacteria All of the above.

Figure 3.33 A mutation occurs, and cytosine is replaced with adenine. What impact do you think this will have on the DNA structure?

GMOs are created by. generating genomic DNA fragments with restriction endonucleases introducing recombinant DNA into an organism by any means overexpressing proteins in E. coli all of the above

Gene therapy involves: a. the introduction of recombinant proteins into individuals b. cloning human genes into plants c. the introduction of a normal gene into an individual carrying a mutant copy d. DNA fingerprinting e. none of these

After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.

Gene therapy can be used to introduce foreign DNA into cells. for molecular cloning by PCR of tissues to cure inheritable disease all of the above

Genetically Modified Foods The creation of transgenic crop plants using recombinant DNA methods involves the transfer of just one gene or a small number of genes to the plants, in contrast to classical breeding methods in which hundreds or even thousands of genes are transferred at once. Explain why this is true. If fewer genes are transferred during the creation of transgenic crops, why are some people afraid that they are dangerous?