If under low power you estimate that five similar cells would fit across the field of view, the size of each cell is approximately I 900 µm. 90 μη . 3.6 mm. 360 µm.

In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.

Compare the two (or more) quantum dots emission at850 pbs/cds.. lnGaAs materials - which would be preferable for labeling certain molecules/organelles in biological samples for fluorescence microscopy? What were your considerations for this choice?

Biology Question

getty Ed Res • You view this cell with the 40x objective lens and it is 7 optical units wide. Use the table below to determine the size of this cell. 1 ocular unit = Objective lens 25 um 4 x 10 μη 10 x 2.5 um 40 x 100 x 1 µm

When the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (0.2% yeast) into each of two new 15 mL centrifuge tubes. Add 200 μL of a 10% (w/v) Sodium Azide*** in YGM solution to one of the 15 mL tubes with yeast suspension. Add 200 μl of YGM to the other 15 mL tube of yeast suspension for your Control. Let both tubes incubate at room temperature for 30 min, vortexing every ~5 minutes What is the % concentration of azide in this sample during metabolic inhibition?What is the % concentration of yeast in this sample during metabolic inhibition?

On the graph that relates absorbance to flavin concentration, draw a horizontal line that starts at the point on the y-axis that corresponds to the absorbance of your unknown sample and ends at the curve you drew through your data points . Next draw a vertical line that starts at the point where the horizontal line intersected the curve and ends at the x-axis. The point where this vertical line intersects the x-axis corresponds to the concentration of flavin in your unknown sample. What was the letter on the tube with your unknown sample? What is the concentration of the flavin in your unknown

Please answer both questions A and B. Question b needs drawing so clearly draw what is given. An illustration (IMAGE 2) can be use as guide

Which molecules, or function groups, or complexes that may be responsible for the absorbance at 595 and 280 wavelengths?    The instrument used is spectrophotometry  Determination of protein concentration ( Bradford-Spectrophotometry) Lab

After trypsinization of cells in the flask and cell counting, you determined a concentration of 10 million cells/mL. What volume of this cell suspension will you use to prepare a 20 mL cell suspension (200,000 cells/mL) for a 24-well plate? O a. 0.4 ul b.0.4 mL Oc. 40 mL O d.40 µL e. none of the above