Use your knowledge of field diameter to calculate the diameter of a Paramecium that takes up 1/3 of the field diameter when viewed with the 40x objective. The field diameter with a 4x objective is 5.3 mm

If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIU

Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope.          c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.

Calculate an estimated IC50 value . ( agent concentration that inhibits colony formation by 50%) show full working.