Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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- Calculate the reduction potential of cytochrome a3 (°' = 0.385 V) at 25.0°C when the Fe3+ form = 87.0 μM and the Fe2+ form = 25.0 μM. 0.440 V 0.353 V 0.417 V 0.387 Varrow_forwardCalculate AGo' for the reaction NADH + pyruvate → NAD+ + lactate You are given: Faradays constant F = 96.5 kJ/mol.V Eo' : NAD+ / NADH = 0.320V Eo' : Pyruvate/lactate = -0.185 V A. 13 kJ/mol В. 97 k j/mol С. 49 kJ/mol D. 26 k j/mol Е. None of the above B.arrow_forwardThe enzyme lactate dehydrogenase catalyzes the reversible reaction lactate (CH3 CHOHCOO )+NAD* рyruvate (CH3 COCOO) + NADH + H+ Given the standard reduction potentials CH3 СОСОО + 2H+ + 2е CH3 CHОНСО" E°'= -0. 185 V NADŤ + H+ + 2e- → NADH = -0. 320 V E°' would you expect the reaction to be spontaneous in the forward or reverse direction under biochemical standard state conditions? How could you change the reaction conditions to force the reaction to be spontaneous in the opposite direction? Please explain your answer.arrow_forward
- Your current Vmax (4x10-3 A/S) from the Michaelis-Menten plot has the units of A/s and was determined using 0.1 mg of ADH in a total volume of 1.25 mL. Use the Beer-Lambert equation followed by simple conversion to express your estimated Vmax in (mol )/s per 1 mg ADH. The molar absorptivity of NADH at 340 nm is 6220 L/(mol*cm).arrow_forwardRefer to the figure shown here, and determine the value of E for the overall oxidation/reduction reaction (refer to the book/lecture slides if you need help with the overall reaction). 11/12102 + 2H* + 2e¯ → H₂0 NAD + H* + 2e → NADH O-1.136 volts 0.496 volts +1.136 volts -0.496 volts EU (volts) – 0.320arrow_forward7. Representative values of V and ApH for the inner mitochondrial membrane and the thylakoid membrane at 25°C are provided in the table. ApH 1.0 pmf Inner mitochondrial 0.166 V membrane Thylakoid membrane 0.03 V 2 A) Calculate the pmf for each membrane and enter the values in the table.arrow_forward
- The table below provides kinetic information when ADH is reacted with ethanol alone, NAD+ alone, and ethanol with NAD+ For the experiment that reacted both ethanol and NAD+ with ADH, estimate Vmax and KM for ADHarrow_forward7. Representative values of Vm and ApH for the inner mitochondrial membrane and the thylakoid membrane at 25°C are provided in the table. Vm Арн 1.0 pmf Inner mitochondrial 0.166 V 0.2251 membrane Thylakoid membrane O,1483 0.03 V B) What is the value of AG for the thermodynamically feasible (“downhill") movement of 1 mole of H across the inner mitochondrial membrane?arrow_forwardBy what factor is sample A2 more concentrated than sample A1?arrow_forward
- Refer to the figure shown here, and determine the value of E for the overall oxidation/reduction reaction (refer to the book/lecture slides if you need help with the overall reaction). 121/202 + 2H+ + 2e → H₂0 NAD + H* + 2e → NADH O-1.136 volts O 0.496 volts O+1.136 volts voltsm -0.496 volts EU (volts) +0.816 - 0.320arrow_forwardConsider the following equilibrium at 298 K. R=8.314 J/K-mol Acetyl-CoA + Oxaloacetate + H20 Citrate + COASH + H* Which one of the following is the correct value for K'eg ifAG" = -32.2 kJ/mol? O 1.11 0.987 1.01 x 10-13 13.0 9.92 x 1012 2480 4.42 x 105 2.27 x 10-6 1.01 -2480 2.56 -13.0 1.08 -0.0130 0.0130 0.926arrow_forward1. Determine the Michaelis-Menten parameters of Vmax and Kỵ for the reaction S+E E.S E.S+W P+E The rate of reaction is given as a function of substrate: [S] 0.2 0.02 0.01 kmol/m³ -I'S kmol/m³.s E.S k₂E+S 0.54 0.28 0.19 0.005 0.1 0.002 0.045arrow_forward
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