If the length of E. coli DNA is 1.36 mm, can you calculate the number of base pairs in E.coli?

To test whether you understand the processes involved in the Central Dogma of Molecular Genetics, determine what amino acid will be formed from the given  DNA strand below:                         #1:  3’   T   A  C   A   T   G   C   C   G   A   A   T   G   C   C   5’                         #2:  3'   T  A   C   T   G  G   C   A    T   A    A   C   A   C   T   5' Note: Prepare the partner strand of the given DNA.  Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA.  Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain.

How much 340bp insert DNA should be added to a ligation in which 50ng of 4.3kb vector will be used? The desired vector: insert molar ratio needs to be 1:3.

For the given restriction enzyme: BslI (CCNNNNN^NNGG)  i) Approximately how many fragments would result from digestion of the human genome (3 × 109 bases) with the enzyme?   ii) Estimate the average size of the pieces of the human genome produced by digestion with the enzyme.  iii) State whether the fragments of human DNA produced by digestion with the given enzyme would have sticky ends with a 5′ overhang, sticky ends with a 3′ overhang, or blunt ends.  iv) If the enzyme produces sticky ends, would all the overhangs on all the ends produced by that enzyme on all fragments of the human genome be identical, or not?   The recognition sequence on one strand for the enzyme is given in parentheses, with the 5′ end written at the left. N means any of the four nucleotides; R is any purine—that is, A or G; and Y is any pyrimidine—that is, C or T. ^ marks the site of cleavage. Note that the recognition sites containing Ys and Rs are not always rotationally symmetrical.

What is the base sequence, specified in the 5′-to-3′ direction, for a segment of newly formed DNA if it was formed using the following template DNA segments?  3′ AATGC 5′  5′ AATGC 3′  3′ GCAGC 5′  5′ GCAGC 3′

Given the Percentage Composition of One Nucleotide ina Genome, Can We Predict the Percentages of the OtherThree Nucleotides?

How would you estimate the size of the unknown DNA fragement just by looking at the gel?

For the following short sequence of double stranded DNA and the given primers, there will be one major duplex DNA product after many cycles (imagine 10 cycles) of PCR.   Provide the sequence of this one major duplex product and label the 5’ and 3’ ends of each strand.  Sequence to be amplified: 5’- GGTATTGGCTACTTACTGGCATCG- 3’ 3’- CCATAACCGATGAATGACCGTAGC- 5’ Primers:  5’-TGGC-3’   and 5’-TGCC-3’

What is the sequence of the unknown DNA sample? (You should give the 5' and 3' ends). Use the gel sequence given below and write also the ingredients used in manual DNA sequencing. Use an empty paper and upload your drawing. || A - T C 0 ||||

The E. coli genome has a superhelical density in vivo of about 0.06. Assuming the DNA has 10.5 bp/turn, what is the expected writhing number of the E. coli genome?