If the length of E. coli DNA is 1.36 mm, can you calculate the number of base pairs in E.coli?

Q: How many adenine bases are there if there are eight thousand cytosine bases in a fifteen thousand bp…

A: Question - How many adenine bases are there if there are eight thousand cytosine bases in a fifteen…

Q: The BamH1 enzyme comes at a concentration of 100,000 U/ml. You are asked to digest 20 ug of DNA with…

A: The term "specific activity" describes the amount of an enzyme's enzymatic activity that is produced…

Q: one should run the following

A: Pairwise sequence alignment is one form of a sequence alignment technique, where we compare only two…

Q: In Table 7-1, why are there no entries for the first fourtissue sources? For the last three entries,…

A: DNA or deoxyribonucleic acid is a type of biomolecule. It is a polymer of deoxyribonucleotides…

Q: The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10° D. In your answers,…

A: The molecular mass of Escherichia coli DNA genome is 3.1 × 109 Daltons. The average molecular weight…

Q: Draw the full structure of the DNA dinucleotide C-T. Identify the 5′ and 3′ ends of this…

A: Deoxyribonucleic (DNA) acid is a double helix structure, which is composed of nucleotides joined by…

Q: A DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than…

A: Friedrich Meischer discovered nucleic acids from the nuclei of pus cells and coined the term…

Q: How many microliters of the pGLO plasmid do you need if you want 5 micrograms of DNA and the plasmid…

A: formula used :  DNA in ug =  (concentration of DNA / pGLO plasmid in ug/ul) ×(volume of DNA in ul)

Q: Below are 9 possible primer pairs. Determine which primer pair is the best choice by considering the…

A: Introduction :- Primers are short oligonucleotide sequence which are required for starting or…

Q: You are working in a biotechnology lab and are analyzing DNA. You obtain a sample of a short…

A: DNA is present inside the nucleus and consists of instructions for the synthesis of protein. The…

Q: A facility says they need 15 μL of a 40 ng/μL solution of plasmid DNA for sequencing. The typical…

A: Appropriate amount of DNA is one the key elements in gene sequencing. Amount of DNA for sequencing…

Q: Using the first and second base key below, predict the DNA sequence given by the SOLID color…

A: Answer:- Nitrogenous bases are crucial components of nucleotides, which are the building blocks of…

Q: In addition to the standard base-paired helical structures, DNA can form X-shaped hairpin structures…

A: Cruciform DNA is a form of non-B DNA that requires at least a 6 nucleotide sequence of inverted…

Q: To carry out sequencing, you need to include 600 ng of DNA and a total of 6.4 pmol of sequencing…

A: DNA, or deoxyribonucleic acid is a long double-stranded molecule that carries the genetic…

Q: Minghao, Mingyu's laboratory partner, was assigned to work on a double-stranded DNA (ds-DNA) sample…

A: DNA is the genetic material present in our body. DNA structure is right handed double helix in which…

Q: What does the symbol “N” indicate (see the arrow)? Is this a problem for getting an accurate DNA…

A: Sequencing of DNA refers to the biochemical methods for determining the order of the nucleotide…

Q: Given a primer sequence 3’- CTGGGAAACATTCTGGAT - 5’, compute for the melting temperature of this…

A: Melting temperature of primer can be defined as a temperature at which one half of the DNA double…

Q: As a result of the Human Genome Project, we learned that approximately % of "junk" DNA is actually…

A:

Q: in the DNA of certain bacterial cells, 13% of the nucleotides are adenine. What are the percentages…

A: Deoxyribonucleic Acid (DNA) is a double-stranded,  right-handed helical molecule that is twisted…

Q: consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. if the given DNA sample was…

A: Ion Torrent sequencing is also known by the name of: Ion semiconductor sequencing pH-mediated…

Q: 5. The A DNA used for digestion was provided at a concentration of 2ug/ul. How much DNA (in…

A: DNA Should be free of contaminants such as phenol, chloroform, alcohol, EDTA, detergents or…

Q: a)Dr. Thisisaneasyexam decides to amplify a gene from a plasmid using PCR. She starts out with 6.6 x…

A: Sure, let me provide a more detailed explanation for the calculations.a) Calculating the number of…

Q: How would the appearance of your DNA gel change if a 0.1% agarose gel slab was used? What about a 7%…

A: If 0.1% agarose gel slab was used then DNA cannot be seen clearly, it will break.Low…

Q: The DNA of a deletion of alpha bacteriophage has a length of 15 micrometers instead of 17…

A: The bacteriophage is those viruses that have the capability to infect bacteria and reproduce inside…

Q: That's the result of Gel electrophoresis of genomic DNA ( Of genomic DNA extraction experiment),…

A: Agarose gel electrophoresis is mainly used for nucleic acid separation due to its large pore size…

Q: How does the 1200-μm-long chromosome of E. coli fit into a bacterial cell 2.0 * 0.5 μm long?

A: Escherichia coli is a Gram-negative, rod-shaped bacterium. It is a facultative anaerobe that is…

Q: You have a very concentrated sample of DNA which you need to dilute for sequencing 1:6000 with a…

A: Dilution is defined as the ratio of volume from stock to the final volume.  Dilution= Stock volume /…

Q: You dilute 10 µL of your isolated DNA with 90 µL of water. You then measure and record the…

A: To calculate the concentration of the plasmid DNA in μg/mL, we can use the following…

Q: A 13-nucleotide section of an autoradiogram from a Sanger sequencing experiment is depicted in the…

A: The DNA is made up of nucleotides that contain nitrogenous bases, pentose sugar and phosphate group.…

Q: The E. coli genome contains 1009 Chi sequences. Do these sequences occur at random, and, if not, how…

A: Recombination is the process of genetic reshuffling where the genetic material is shared within or…

Q: 5'- CTC GAG CGA AG was obtained as a dry solid. The sample was dissolved in 1000 ul of deionized…

A: Deoxyribonucleic acid (DNA) is a complex organic molecule found in all prokaryotic and eukaryotic…

Q: Visually compare the intensity of your team’s Plant-DNA sample with the intensity of the 3 kb band…

A:

Q: Recombinant protein production by a genetically modified Escherichia coli strain is proportional to…

A: In this scenario, we explore aspects of recombinant protein production using a genetically modified…

Q: r DNA isolation using Qiagen extraction kit, the ophotometric measurements. Explain the situation i)…

A: The deoxyribonucleic acid extraction method frees deoxyribonucleic acid from the cell then}…

Q: If the bandicoot genome is 3.62 x 109 base pairs, and the "highly repetitive DNA" fraction is…

A: The genome is the entire DNA content (in base pair) of a haploid (n) cell. That means in case of a…

Q: please solve this with step-by-step calculations and explanations.

A: Here is handwritten answer

Q: 1) You wish to make a restriction map of a 17.0 kb linear fragment. You digest the fragment with…

A: Restriction map is a map of the restriction sites present in gene/DNA sequence. Restriction enzymes…

Q: Rice genome contains 30% C on a molar basis. What are the mole percentages of A, G, and T?

A: In a double helical DNA, there are 4 nucleotides are found ATP, GTP, CTP, TTP. In this double…

Q: What are the consequences for a DNA sequencing reaction if the ratio of dideoxyribonucleoside…

A: Introduction  DNA is composed of nucleotides arranged in a specific sequence. Prior knowledge of DNA…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.