If photosynthesizing plants are grown in thepresence of 14CO2, is every carbon atom of the glucose that is produced labeled with the radioactive carbon? Why or why not?
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If photosynthesizing plants are grown in the
presence of 14CO2, is every carbon atom of the glucose that is produced labeled with the radioactive carbon? Why or why not?
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- The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)The herbicide dinoseb, which was removed from the market many years ago, killed plants by uncoupling oxidative phosphorylation. Ironically, plants have embedded in the inner membrane of their mitochondria a protein named the “uncoupling protein” that essentially can carry out the same process caused by dinoseb. The mechanism of uncoupling oxidative phosphorylation involves which of the following scenarios. A. Excess electrons oxidize lipids in the mitochondrial membranes, thus destroying the electron transport chain B. Excessive oxygen is consumed via reduction to water, thus depriving the mitochondria of the ability to carry out phosphorylation that requires oxygen C. The membrane selectivity required to maintain a proton gradient is altered so the potential for driving ATP synthesis via chemiosmosis is degraded D. Uncoupling oxidative phosphorylation kicks a cell into a fermentative pathway that cannot supply sufficient ATPThe first amino acid in protein synthesis in the chloroplast is N-formyl methionine. What is the significance of this fact?
- Why does the presence of proteorhodopsin enable phototrophy but not photosynthesis by SAR11?The photosynthetic process used by the green sulfur bacteria, under anaerobic conditions, is best represented by which of the following balanced equations? cell bio 6CO2 + 12H2S C6H12O6 + 6S2 + 6H2O C6H12O6 + C6H12O6 C12H22O11 + H2O 6CO2 + 12H2O C6H12O6 + 6O2 + 6H2O C12H22O11 + H2O C6H12O6 + C6H12O6 C6H12O6 + 6O2 + 6H2O 6CO2 + 12H2OGlyceraldehyde 3-phosphate dehydrogenase in chloroplasts uses NADPH to participate in the synthesis of glucose. In gluconeogenesis in the cytoplasm, the isozyme of the dehydrogenase uses NADH. Why is it advantageous for the chloroplast enzyme to use NADPH?
- In cyclic photophosphorylation, it is estimated that two electrons must be passed through the cycle to pump enough protons to generate one ATP. Assuming that the AG for hydrolysis of ATP under conditions existing in the chloroplast is about –50 kJ/mol, what is the corresponding percent ef- ficiency of cyclic photophosphorylation, using light of 700 nm?Explain why both starch synthesis and the activity of the Triose-P Phosphate translocator serve to maintain the ability of the chloroplast to maintain high photosynthetic rates.Example 14.6 The oxygen requirement for cell growth in glueose can be represented by the following equation (Mateles, 1971) 32NC+8(Nh2)+16(No2) Fx/sM +yo2 – 2.67yc + 1.714YN2 – 8y H2 ro2 = In which ro2 is the oxygen required for each gram of cells produced, N stands for no. of atoms present in each molecule substrate, y stands for mass fractions and M is the MW of the substrate. The yeast cell may be considered to be CH1.800.5NO.2. Calculate rO2 if the yield factor (Yx/s) is 0.46g of cells produced for each gram of substrate consumed.
- Of course, one cannot stick a piece of tape to each carbon atom in the actual glucosemolecule. But one can do the next best thing: use radioisotopes to trace themovement of various atoms. Specifically, because carbon dioxide is a gas, usingradioactive C-14 allows for the easy detection of the 14CO2 that is given off.Suppose you introduce one glucose molecule that has had all of its carbons radioactivelylabeled. Suppose further that each step in glycolysis and in the citric acid cycle takes oneminute to complete (so glycolysis, for instance, takes ten minutes to complete, theconversion to acetyl CoA takes one minute, and the citric acid cycle takes eight minutes).Draw a plot of time (x-axis) versus radioactivity detected by a gas sensor (y-axis). Labelthe x-axis with enough time increments so it is clear at what minute the radioactivitywas detected. Use differing peak heights to give an indication of the amount ofradioactivity detected at that point.We humans do not express an alpha-Galactosidase enzyme and therefore cannot break down stachyose but the bacteria that live in our large intestine can. These bacteria contain an alpha-Galactosidase enzyme that hydrolyzes the galactopyranosyl-alpha (1-6) glycosidic bonds. The bacteria then ferment the products to generate the gas that is famously associated with eating beans. Draw the Haworth projections of the products of alpha-Galactosidase catalyzed reaction. Name the products of this enzyme catalyzed reaction.What is the role of the aspartate 118 residue in the Human inorganic Pyrophosphatase mechanism? include a drawing of the mechanistic proposal.