If isolated mitochondria are incubated with a source of electrons such as succinate, but without oxygen, electrons enter the respiratory chain, reducing each of the electron carriers almost completely. When oxygen is then introduced, the carriers become oxidized at different rates (Figure Q14–1). How does this result allow you to order the electron carriers in the respiratory chain? What is their order?

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter23: Protists
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Problem 33CTQ: Without treatment, why dees African sleeping sickness invariably lead to death?
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If isolated mitochondria are incubated with a
source of electrons such as succinate, but without oxygen,
electrons enter the respiratory chain, reducing each of the
electron carriers almost completely. When oxygen is then
introduced, the carriers become oxidized at different rates
(Figure Q14–1). How does this result allow you to order the electron carriers in the respiratory chain? What is their
order?

100
add
O2
Figure Q14-1 Rapid
spectrophotometric
analysis of the rates of
oxidation of electron
carriers in the respiratory
chain (Problem 14-7).
Cytochromes a and as
cannot be distinguished
and thus are listed as
50
add
succinate
(a + a3)
time
cytochrome (a + a3).
reduced cytochrome (percent)
Transcribed Image Text:100 add O2 Figure Q14-1 Rapid spectrophotometric analysis of the rates of oxidation of electron carriers in the respiratory chain (Problem 14-7). Cytochromes a and as cannot be distinguished and thus are listed as 50 add succinate (a + a3) time cytochrome (a + a3). reduced cytochrome (percent)
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