if during serial dilution, you consistently had an air bubble in your pipet and did not deliver the correct amount of dye for dilution how can it affect the extinction coefficient found from the calibration curve?
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if during serial dilution, you consistently had an air bubble in your pipet and did not deliver the correct amount of dye for dilution how can it affect the extinction coefficient found from the calibration curve?
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- If while performing the serial dilution, you consistently had an air bubble in you pipet and di not deliver the correct amount of dye for dilution. How would this affect the exctinction coefficient found from the calibration curve? ExplainIn UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be specific (4-5 lines) .
- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?In zone electrophoresis, why is it possible to isolate the components of a sample and recover them for identification? a) what influences the direction of migration of the components of a sample in zone electrophoresis?
- Why is UV light used in Column chromatography experiment ?Differentiate the “hot method” and “cold method” of acid-fast stainingYou saw no color change in both the first step and second step of the Nitrate Reduction test. What is the result of this experiment? Also, explain what happens when an excessive amount of zinc is added in the second step of this test.
- A strep culture is sampled at two points in time, 1 hour and 10 minutes apart. 3.2 x 105 cells/ml are found in the first sample, and 1.8 x 107 cells/ml in the second sample. What was the generation or doubling time (in minutes)?Suppose your professor handed you a bottle of kombucha with 2.0 ml in it and told you to make a 10^-2 dilution of the entire culture. Explain how you would do this. Show your calculations.How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
