If an octapeptide IDEWLVAS is digested with chymotrypsin enzyme, which column would you use? Chymotrypsin cleaves on the carboxy side of bulky and aromatic amino acids. A. Sephadex column B. None of the above C. Anion Exchange column D. Cation Exchange column Please explain why.
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If an octapeptide IDEWLVAS is digested with chymotrypsin enzyme, which column would you use?
Chymotrypsin cleaves on the carboxy side of bulky and
A. Sephadex column
B. None of the above
C. Anion Exchange column
D. Cation Exchange column
Please explain why.
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- Define the following terms: a. respiratory control b. glycerol-3-phosphate shuttle c. uncoupling protein d. oligomycin e. malate–aspartateWhich of the following amino acids would be first to elute at pH 6.5 from a cation-exchange column? A. arginine B. alanine OC. glutamine D. glutamic acid O E. glycineDraw the chemical structures resulting from the transamination of the following amino acids: a. Aspartic acid + alpha-ketoglutarate à ______________ b. Glutamic acid + pyruvate à ______________________ c. Alanine + alpha-ketoglutarate à ___________________ d. Phenylalanine + pyruvate à ______________________ e. Serine + alpha-ketoglutarate à ____________________
- D-Gluconic acid is formed from D-Glucoge Select one: a. By reduction b. By oxydation c. By substituton d. By esterificationDefine the following terms: a. lactone b. aldaric acid c. aldonic acid d. uronic acid e. epimerizationFigure 1 shows the catalytic triad of a-chymotrypsin. Identify A, B and C. Describe the subsequent steps of stage 1 and stage 2 of a- chymotrypsin mechanism. Illustrate with diagrams. A B CH2 H-N N: H-O Figure 1
- Consider the decapeptide angiotensin I.a. What products are formed when angiotensin I is treated with trypsin?b. What products are formed when angiotensin I is treated with chymotrypsin?c. Treatment of angiotensin I with ACE (the angiotensin-converting enzyme) cleaves only the amide bond with the carbonyl group derived from phenylalanine to afford two products. The larger polypeptide is angiotensin II, a hormone that narrows blood vessels and increases blood pressure. Give the amino acid sequence of angiotensin II using three-letter abbreviations. ACE inhibitors are drugs that lower blood pressure by inhibiting the ACE enzymeAlkaptonuria is a rare organic aciduria involving a deficiency in homogentistic acid oxidase resulting in accumulation of homogentisic acid which is an intermediate in the degradative pathway of which of the following amino acids? a. Threonine b. Alanine c. Tyrosine d. ValineWhat would happen to the functionality of cholic acid if 3 additional hydroxyl groups were added to the side of the molecule that usually has none? (In other words, there would be 6 hydroxyl groups evenly distributed around the molecule. A. There would be more thorough (better) digestion of fats. B. It would mix with the watery phase of chyme. C. It would mix with lipids in the fatty phase of chyme. D. Chemical digestion of lipids would be less effective. E. Both choices A and B are correct. F. Both choices B and D are correct.
- Which of the following amino acid residues would not participate in general acid-base catalysis? A. glycine histidine B. C. aspartate D. glutamic acid E. lysineCholestyramine is an anion-exchange resin used by clinicians to lower serum cholesterol in patients. This oral medication forms insoluble complexes with bile salts in theintestine, thus preventing their reabsorption. Explain themechanism by which cholestyramine lowers serum cholesterol levels.From the three figures below, indicate which represents best the catalytic pocket of trypsin, a serine protease, secreted by acinar pancreatic cells. C) A) A. Figure A. B.Figure B. ⒸC. Figure C. Asp 189 B) Asp 189 Val 190 Val 216 work of Val 216 Val 190