If a slice of pancreatic tissue had been incubated in [ 3 H]leucine continuously for 2 hours, where in the acinar cells would you expect to find incorporated radioactivity
Q: Emodin is characterised by: Select one: a. It reacts with alkali and give a red color b. It react…
A: Emodin It is a chemical compound, which belongs to the anthraquinone family. It isolated from…
Q: Given that both human cells and pathogens synthesize proteins at ribosomal sites, how can…
A: Microbes are significant because they have shaped the history of our planet. Some microorganisms are…
Q: If my saliva tested positive for starch. How would that affect the interpretation of the results for…
A: Saliva Saliva is the gland located in the mouth which secret salivary amylase in the mouth.…
Q: Why should the cell density of the inoculum be as small as possible? Why can we not standardize the…
A: The process of introduction of microbes into a nutrient media or other similar environments which…
Q: Please answer both questions asap. Thank you. - Why would SV40 cause the culturing to occur of HMLE…
A: 1. SV40 three genes encoding SV40 large T antigen , telomerase catalytic subunit and H Ras…
Q: Four proteins Cytochrome C (pI=10.2) Myoglobin (pI=7.2), Hemoglobin (pI = 6.8) and Serum Albumin…
A: Introduction: Proteins are important macromolecule that helps in the structure and function of the…
Q: Which bacteria is demonstrating the presence of tryptophanase? None of the bacteria Bacteria S…
A: In indole production test, we determine the microorganism's ability to produce an enzyme…
Q: What is the mechanism of the reaction of the Bradford reagent with proteins? (explain in not less…
A: The Bradford protein assay is used to measure the concentration of total protein in a sample.
Q: holsappleeus scarius is a normal flora of the human intestenal tract. its facultative anaerobe…
A: Gut microbial flora is found in three primary regions. They are the stomach, colon, and small…
Q: What is the purpose of adding ice-cold ethanol to the cell lysate? What is the purpose of…
A: the answer is with respect to the DNA isolation techniques:
Q: 1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes…
A: Those exploring chemical characteristics and analyzing reactions, in general, utilize volumetric…
Q: Can the resazurin reduction test be used to determine whether a milk is properly pasteurized or…
A: Pasteurization is a process that helps in keeping the milk fresh and helps in avoiding the…
Q: Of, Bacillus megaterium, Bacillus sphaericus, and Bacillus subtilis, which are positive for lipase…
A: Bacillus megaterium - rod-like, Gram-positive, mainly aerobic spore-forming bacterium Bacillus…
Q: Discuss the concept of microwave fixation and cryofixation, and why it is suitable for…
A: Microwave fixation and cryofixation are the bilogical techniques which are used for stabilising th⁶e…
Q: Why are platelets not considered cells? And why does it contain a kernel?
A: Platelets, or thrombocytes, are tiny, colorless cell particles in our blood that form clots and hold…
Q: Which of the following are positive for the production of indoor from the amino acid tryptophan?
A: indole is produced by reductive deamination from tryptophan through the intermediate molecule indole…
Q: Can you specifically verify which one is canola oil, oleic acid, pork lard, lecithin, and lauric…
A: Lipids Lipids are defined as organic compounds that are strictly insoluble in water and are only…
Q: Describe the appearance of the protein solutions in each of the tubes after addition of the…
A: Proteins are complex nitrogen-containing organic molecules that involve all aspects of the live…
Q: Draw the products formed when -D-idose is treated with C6H5NHNH2 (excess), H+
A:
Q: For which of the five hydrolysis tests (starch, casein, triglycerides, tryptophan, and urea) would a…
A: Propionibacterium acnes is a Gram-positive, rod-shaped bacterium. It is an aerotolerant anaerobic…
Q: What do you predict would happen if the lipase was boiled at 100°C? What do you predict would happen…
A: Enzymes are special kinds of proteins that catalyze all biochemical reactions in the body. Lipase is…
Q: Which of the the following results of the extracellular enzyme tests is FALSE? Question 3…
A: Answer: Extracellular Enzymes or Exoenzymes are the enzymes secreted by the organisms or cells and…
Q: How does horseradish peroxidase (HRP) contribute to creating the final colored sample? O A. HRP is a…
A: In biochemistry techniques, the enzyme horseradish peroxidase (HRP), which is isolated from the…
Q: What diagnostic tests will benefit from collecting both a blood sample and a urine sample from a…
A: Note: Please upload other questions separately. Answer: Introduction: A primary urine test can…
Q: Which of the following pairs are not correctly matched? Select one: a. Transient flora - removed…
A: Good hand hygiene plays important role in preventing infection transmission and protects from…
Q: Bacillus thuringiensis forms protien crystals which contain insectidical protein , what does this…
A: Bacillus thuringiensis (Bt) is a bacteria that is usually found in soil. It is a gram-positive…
Q: What diagnostic tests will benefit from collecting both a blood sample and a urine sample from a…
A: The tests are used to diagnose the disease condition and treat those disease condition at their…
Q: Enzyme chymotrypsin (enzyme digest) Samples: - 1 & 5- Standard Protein (Ladder) - 2 & 6- UNCUT…
A: Hypothesis: if similar bands are obtained for similar treatment then protein is structurally similar…
Q: The following laboratory test results were obtained in a patient with severe jaundice, right upper…
A: The yellowish pigment that is a result of red blood cell breakdown is bilirubin. Liver process the…
Q: While performing the experiment " Detection of ammonium sulphate in Milk", a pink color result is…
A: The risk of adulteration is very common in milk. These adulterants can be milk from different…
Q: B indicates substrate and C indicates 0.1g/L (concentration) In this case, is B manipulated…
A: The Scientific method is a process with the help of which scientists try to investigate, verify, or…
Q: If saliva alone tests positive for either starch or glucose in both Benedict's Test and Iodine Test,…
A: Benedict's test is used to test reducing sugars . These include monosaccharides such as glucose and…
Q: Salivary Digestion: Procedure Starch – saliva solution added to iodine solution Observations…
A: Sugars or carbohydrates are the most abundant biopolymers in living organisms. Carbohydrates are…
Q: The following laboratory test results were obtained in a patient with severe jaundice, right upper…
A: Jaundice is a physiological condition where the serum levels of bilirubin are higher than normal. As…
Q: 2.2 An investigation was carried out to determine the effect of a detergent containing fat-digesting…
A: 2.2 Enzymes act as biological catalysts. They fasten reactions and increases the rate by lowering…
Q: what steps will be appropriate if the cavity has only 0.5mm of remaining dentin before entering the…
A: Dentin is the part of the tooth that is beneath the enamel and cementum. It contains microscopic…
Q: Why might you expect a small dose of puromycin to be less effective in repressing bacterial growth…
A: Introduction: Bacteria are little single-celled organisms. These microbes are discovered wherever on…
Q: Before using the API® 20E System, what test must be performed to confirm the identity of your…
A: Bacteria are of two classes gram-negative and gram-positive bacteria. They are classified on the…
Q: How do HABA, salicylate, and acetylsalicylic acid bind to BSA? Why do the absorbances for each of…
A: In this,salicylate to be and acetylsalocyclic acid to be inhibitors of HABA-BSA binding. from…
Q: What is the Bench Side Biochemical Test for Enterococcus faecalis and Proteus mirabilis?
A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…
Q: What is the relevance of the use of sugar, spice, and starch in the detection of sporeformers?…
A: Note: According to the guidelines, we are answering questions 2 and 3 only as the specific question…
Q: Can lactic acid culture infect the human gastrointestinal tract? If yes, why is this method not used…
A:
Q: Loop of Henle main fuction is ?
A: Loop of Henle is U-shaped tubule (hair-pin configuration) in each nephron (structural unit of…
Q: Write down a comparison of the sizes of the untreated Allium cepa cell and the colchicine-treated…
A: Onion, or Allium cepa, is a culinary and medicinal spice from the Liliaceae family that is used all…
Q: Why are mint flavored foods containing menthol perceived as cold and foods containing the chemical…
A: All the sensation in the body are sensed by receptor which generates action potential which is…
Q: Of, Bacillus cereus, Bacillus megaterium, Bacillus sphaericus, and Bacillus subtilis, which are…
A: Lipases are enzymes that catalyze the degradation of fats and oils and convert them I to fatty acids…
Q: If saliva alone tests positive for either starch or glucose, how would that affect the…
A: Our body has various digestive juices or enzymes that facilitate the digestion process. One such…
If a slice of pancreatic tissue had been incubated in [ 3 H]leucine continuously for 2 hours, where in the acinar cells would you expect to find incorporated radioactivity?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 2 images
- A 4-month-old baby is admitted to the hospital with a temperature of 102 F (38.9 C). Why is it critical to treat the fever as quickly as possible?Regarding the use of animal cells in culture, it is correct to state that: * (a) Low cost culture media composed of mineral salts and amino acids are used (b) Cells must be kept in semi-solid media, allowing them to come into contact with the atmosphere rich in carbon dioxide. (c) Greenhouses with controlled temperature and atmosphere composed predominantly of carbon monoxide are used (d) On an industrial scale, culture media supplemented with serum of animal origin are used, which makes them media of indefinite composition (e) Culture media must not be sterilized by autoclaving, preferably filtration and/or ionizing radiation methods are usedAdult patient, admitted to a hospital, receives prescription of 25% zinc oxide ointment. Whereas the pharmacy only provided 480 grams of zinc oxide ointment at 10% concentration, how many grams of zinc oxide should be incorporated to prepare the prescribed ointment?
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?You are stumped for a while, because you really don't want to spend all day running columns, and then having to measure the column fractions with UV-VIS. Your colleague however tells you that it is possible to use the spectrum of the mixture to determine the concentration of cach vitamin in the mixture. (c) Briefly explain why it is possible to use the spectrum of the mixture to determine the concentration of each vitamin in the mixture. Now that you realise that it is possible, you get some information about the molar absorptivities of cach vitamin at cach Amax, and list it in Table 1. Table 1. The molar absorptivities of Vitamins B1 and B6 measured in pure samples of each. E (M' cm') a (nm) Vitamin B1 Vitamin B6 423 5075 240 11280 285 1504 (d)(1) Use the information in Table 1 and the absorbance of the sample mixture at the relevant wavelengths (Figure 1) to determine the concentration of Vitamin B1 in the diluted, prepared sample. (d)(i) Determine the mass of Vitamin B1 in one of…You treat cells briefly with radioactively labeled cysteine and then transfer them to a medium with unlabeled cysteine. Where would you expect to detect radioactivity immediately after the transfer to the unlabeled medium? Where would you expect to detect it an hour later?
- Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 1 (1) (ii) 3 (iii) 2 (iv) 4 Note: Count cells on the four labelled squares. Include cells touching the line on top and left. 18P 06. ¿66 Ⓡ Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingWhy sucrose gives negative Benedict test?
- In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a certain depth? Why must the absorbance of the inoculum be standardized?What causes the DNA to migrate from the wells toward the positive electrode? a) DNA is positively charged too, and so was attracted to the positive electrode b) DNA is negatively charged, and so was attracted to the positive electrode c) DNA is polar and forms hydrogen bonds with the positive electrodes d) DNA is electrically charged, and so cycles through an electric field The ratio of absorption at 260nm to absorption at 280nm is commonly used to assess. (Note: purity of DNA should be the right answer, but a is shown as wrong) a) The concentration of DNA and RNA. b) The purity of protein. c) Whether your DNA or RNA is contaminated Trizol. d) The concentration of protein in your sample. How many different bases are associated with RNA? a) 5 b) 4 c) 3 d) 6Refeeding syndrome causes dangerous fluctuations in which of the following? Question 7 options: a) Ketone bodies b) Liver enzymes c) Electrolytes d) Triglycerides