Identify the CORRECT sequence of steps that occurs during every cycle of PCR. 1. The primers hybridize to the target DNA. II. Binding of DNA polymerase to the target DNA. III. Extension of primers to make a copy of the target DNA. IV. The mixture is heated to a high temperature to denature the double stranded target DNA A. I, II, III, IV. B. II, I, IV, III. C. IV, I, II, III. D. IV, II, I, III.

Which of the following natural cellular process does PCR use to amplify a target DNA? A. Gene Expression B. DNA symthesis C. Transcription of DNA D. Translation

36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers bind (anneal) to complementary sequences of the target DNA. 2) DNA is heated to a high temperature to cause denaturation (DNA is single-stranded). 3) DNA polymerase extends the 3’OH of primers to make copies of the target DNA. A) 1, 2 and 3 B) 3, 2, and 1 C) 2, 1, and 3 D) 1, 3, and 2

1. produces multiple identical copies of a gene               [ Choose ]            a. DNA ligase              b. gene cloning            c. gel electrophoresis              d.restriction enzymes